Lalitha John

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The nested PCR assay for the detection of 16S rRNA gene fragment of ehrlichia canis from the blood of dogs was standardized for the first time in India. The genus-specific primers amplified a 477 bp band of Ehrlichia sp. in the first PCR. The nested PCR assay using species-specific primers produced a 387 bp band of e. canis. The nested PCR detected e. canis(More)
Integrins form a major family of heterodimeric cell surface receptors. Individual family members each comprise noncovalently linked, dissimilar, a- and β-subunits. Each subunit is the product of a different gene (1), and a-subunits appear to have evolved separately from β-subunits (2). Seventeen a-, and eight β-subunits are currently described. It is the(More)
A novel in vitro fl ow through technique was developed and evaluated for immunodiagnosis of cystic echinococcosis in cattle using hydatid specifi c non-cross reactive 8 kDa protein. The 8 kDa protein was prepared from hydatid cyst fl uid by DEAE-Sepharose fast fl ow anion exchange chromatography. In this fl ow through technique, the 8 kDa antigen was coated(More)
Anthelmintics are used traditionally as an integral part of helminthic control strategies for grazing livestock to prevent production losses from parasitic infections. The continuous and indiscriminate use of the same anthelmintics over years together as the sole means of control are now failing due to the emergence of resistance strains of helminths.(More)
A novel in vitro flow through technique was developed and evaluated for immunodiagnosis of cystic echinococcosis in sheep using hydatid specific non-cross reactive 8-kDa protein. The 8-kDa protein was prepared from hydatid cyst fluid by DEAE-Sepharose fast flow anion exchange chromatography. In this flow through technique, the 8-kDa antigen was coated on(More)
A 35 kDa midgut polypeptide of rhipicephalus haemaphysaloides was isolated for the first time by immunoaffinity chromatography using immunoglobulin ligands from rabbits immunized with 35 kDa midgut polypeptide. An experimental immunization study was conducted on 12 New Zealand white rabbits using affinity purified 35 kDa midgut antigen (Aff RhGM 35 kDa Ag)(More)
Selected aspects of the ovine immune system were examined during the course of repeated infestations with the ixodid ticks, Haemaphysalis bispinosa and Hyalomma anatolicum anatolicum that naturally infest sheep, either individually or together. By the use of flow cytometry it was shown that total T-lymphocyte numbers were significantly reduced from the(More)
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