Lakkakula Satish

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The effect of seaweed liquid extracts (SLEs) made from Gracilaria salicornia, Padina gymnospora, Padina boergesenii, Gelidiella acerosa and plant growth regulators (PGRs) were examined on in vitro mass propagation using hypocotyls and leaf disc explants of brinjal (Solanum melongena L.) cultivar Pusa purple long. For the germination bioassay, seeds(More)
Effect of plant growth regulators and spermidine on somatic embryogenesis and regeneration was investigated in finger millet. Mature embryos, and 3 days old seedling-derived shoot apical meristems were cultured on Murashige and Skoog (MS) medium containing picloram, 2,4,5-trichlorophenoxyacetic acid, 2,4-dichlorophenoxyacetic acid (2,4-D) and(More)
The effects of cefotaxime, amino acids and carbon source on somatic embryogenesis and plant regeneration using mature seeds in four genotypes (‘CO5’, ‘CO7’, ‘TNAU43’ and ‘RS118’) of foxtail millet have been studied. The ‘CO5’ gave a superior response in callus induction, somatic embryogenesis and regeneration. The highest percentage (69.3%) of embryogenic(More)
A rapid and reproducible protocol for direct plant regeneration has been established using in vitro-derived, actively growing shoot apical meristems (SAMs) of the genotype 'CO(Ra)-14'. Shoot apical meristems from 3-day-old seedlings of three finger millet genotypes viz, 'CO(Ra)-14', 'Try-1', and 'Paiyur-2' were assessed for the efficiency of direct shoot(More)
The scope of the present study is to investigate the effect of plant growth regulators and seaweed liquid extracts (SLEs) on in vitro regeneration of the Ayurvedically important memory plus plant Bacopa monnieri using shoot tips. Sodium alginate-encapsulated shoot tips were cultured on Murashige and Skoog (MS) medium containing 6-benzylaminopurine (BAP),(More)
The effect of seaweed liquid extracts (SLEs) from Gracilaria edulis and Padina boergesenii was studied on finger millet bioassays. Finger millet seeds were germinated on 0–100 % of SLEs. Genotype ‘PR-202’ showed superior response with higher frequency of germination (99.6 %), fresh weight (1.1 mg seedling−1), shoot length (13.4 cm), root number (4.8), and(More)
Agrobacterium rhizogenes mediated transformation has been experimented in leaf explants of the memory herb Bacopa monnieri in order to assess the regeneration potential of hairy roots (HR) followed by the elicitation of transformed plants for increased Bacoside A production. Out of the four strains tested, A4 and MTCC 532 derived HR exhibited regrowth in MS(More)
The present study explores the non-bactericidal anti-virulence efficacy of green synthesized silver nanoparticles (AgNPs) from Gelidiella acerosa against multi-drug resistant Vibrio spp. Spectral characterization of AgNPs was performed through UV-Visible, FT-IR, and energy-dispersive spectroscopic techniques followed by X-ray crystallography and zeta(More)
Genetic variation among 45 genotypes of sorghum (Sorghum bicolor L.) representing seven subpopulations was assessed using three single primer amplification reaction (SPAR) methods viz., inter-simple sequence repeat (ISSR), random amplified polymorphic DNA (RAPD) and directed amplification of minisatellite-region DNA (DAMD). Totally 15 ISSR, 8 RAPD and 7(More)
We have developed an improved Agrobacterium-mediated transformation and rapid regeneration system for four cultivars (‘CO(Ra)-14’, ‘PR-202’, ‘Try-1’ and ‘Paiyur-2’) of finger millet using optimized transformation and direct plant regeneration conditions. The shoot apical meristems (SAMs) were used as explants in this study. Agrobacterium strain EHA105(More)
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