La-ongthong Vajrabhaya

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An evaluation of the accuracy of the "Root ZX" apex locator was conducted on 20 single-rooted teeth that had been extracted because of severe disease or orthodontic treatment. After the pulp chamber had been accessed and the tooth length determined using the Root ZX apparatus, the file was fixed to the tooth with light-cured composite resin. After(More)
This study evaluated the cytotoxicity of four single component dentin bonding agents: Syntac Single Component, Prime & Bond 2.1, Single Bond and One Up Bond F. The test materials were applied on dentin discs of dentin barrier models in the same way as in the clinical procedures recommended by each manufacturer. Cell viability of L 929 after exposure with(More)
A case of "double" dens in dente with an open apex and periapical lesion is reported. Nonsurgical endodontic treatment was performed. The canals were medicated for 5 months with calcium hydroxide before obturation with combined sectional and thermoplasticized warm gutta-percha. Follow-up revealed an asymptomatic patient and a reduction in size of the(More)
OBJECTIVE This study evaluated the cytotoxicity of 3 self-etching bonding systems: Optibond Solo Plus SE primer, Xeno III, and i Bond. STUDY DESIGN The test materials were applied on the dentine discs of dentine barrier models in the same way as in the clinical procedures recommended by each manufacturer. 3-D cell culture of Bovine pulp-derived cells(More)
OBJECTIVE The purpose of this study was to compare the cytotoxicity of 2 gutta-percha solvents, chloroform and GP-Solvent, on cell line L929. STUDY DESIGN 2 gutta-percha solvents were diluted into the concentrations of 1:100, 1:400, and 1:800. The experiment was done in a 96-well tissue-culture plate. Cell viability of L929 was determined after each(More)
The aim of this study was to evaluate the response of a multilayer compared with a monolayer cell culture using six root canal sealers. Both monolayer and multilayer of MU-mu-1 (Mahidol University mouse cell line 1) were cultured in separate 96-well plates. Following incubation at 37 degrees C in 5% CO2 for 4 h in the presence of each sealer, cells were(More)
OBJECTIVE The aim of this study was to evaluate the cytotoxicity of three dentine bonding agents (G-Bond, Clearfil S(3) Bond and Clearfil SE Bond X) in cell-culture perfusion. METHODS In this experiment, 8×10(4) TCPC SV40 cells (bovine-pulp-derived cells transfected with simian virus 40 large T-antigen) in MEM-alpha media, 20%FCS were seeded on mesh in a(More)
The aim of this study was to evaluate Sulphorhodamine-B (SRB) staining against 51Cr-release in cytotoxicity tests of six endodontic sealers, namely, MU sealer (Mahidol University) ROCANAL 2, ROCANAL 3, Apexit, Endomethasone, and AH-26. Monolayers (5 x 10(5) cells/ml) of the mouse cell line Mu-mu-1 were used as test cells. Following incubation at 37 degrees(More)
OBJECTIVE The objective of this study was to evaluate the cytotoxicity of furcal perforation repair materials, GI and MTA, using cell culture technique. STUDY DESIGN The extract of ProRoot MTA and Ketac Molar were treated on PDL cells in a 96-well tissue-culture plate. Cell proliferation after an incubation period of 3 days was determined by using MTT(More)
OBJECTIVES The aim of this study was to compare the results of dimethylthiazol diphenyltetrazolium bromide (MTT) and neutral red uptake (NRU) assays of Clinacanthus nutans cytotoxicity. MATERIALS AND METHODS Mouse fibroblast (L929) cells were exposed to 0.01%, 0.1%, 0.25%, and 0.5% (W/V) C. nutans in a 96-cluster-well-culture plate for 24 h. The cell(More)