La-iad Nakkrasae

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A molecular marker for germ cells of the giant freshwater prawn, Macrobrachium rosenbergii, was studied. A vasa-like gene, Mrvlg, from the ovary was isolated and characterized by a reverse transcriptase-polymerase chain reaction (RT-PCR) method. A full-length sequence was obtained by the rapid amplification of cDNA end (RACE) method. Analysis of the(More)
Zebrafish germ cells contain granular-like structures, organized around the cell nucleus. These structures share common features with polar granules in Drosophila, germinal granules in Xenopus and chromatoid bodies in mice germ cells, such as the localization of the zebrafish Vasa, Piwi and Nanos proteins, among others. Little is known about the structure(More)
Charoenphandhu N, Nakkrasae L, Kraidith K, Teerapornpuntakit J, Thongchote K, Thongon N, Krishnamra N. Two-step stimulation of intestinal Ca absorption during lactation by longterm prolactin exposure and suckling-induced prolactin surge. Am J Physiol Endocrinol Metab 297: E609–E619, 2009. First published June 30, 2009; doi:10.1152/ajpendo.00347.2009.—During(More)
The milk-producing hormone prolactin (PRL) increases the transcellular intestinal calcium absorption by enhancing apical calcium uptake through voltage-dependent L-type calcium channel (Cav) 1.3. However, the redundancy of apical calcium channels raised the possibility that Cav1.3 may operate with other channels, especially transient receptor potential(More)
Prolactin (PRL) was previously demonstrated to rapidly enhance calcium absorption in rat duodenum and the intestine-like Caco-2 monolayer. However, its mechanism was not completely understood. Here, we investigated nongenomic effects of PRL on the transepithelial calcium transport and paracellular permselectivity in the Caco-2 monolayer by Ussing chamber(More)
During pregnancy and lactation, the enhanced intestinal Ca(2+) absorption serves to provide Ca(2+) for fetal development and lactogenesis; however, the responsible hormone and its mechanisms remain elusive. We elucidated herein that prolactin (PRL) markedly stimulated the transcellular and paracellular Ca(2+) transport in the duodenum of pregnant and(More)
Previous investigations suggested that prolactin (PRL) stimulated the intestinal calcium absorption through phosphoinositide 3-kinase (PI3K), protein kinase C (PKC), and RhoA-associated coiled-coil forming kinase (ROCK) signaling pathways. However, little was known regarding its detailed mechanisms for the stimulation of transcellular and voltage-dependent(More)
Prolactin (PRL) is reported to stimulate calcium absorption in the rat’s small intestine. However, little is known regarding its effects on the cecum, a part of the large intestine with the highest rate of intestinal calcium transport. We demonstrated herein by quantitative real-time polymerase chain reaction and Western blot analysis that the cecum could(More)
Besides being a Ca²-regulating hormone, parathyroid hormone (PTH) has also been shown to regulate epithelial transport of certain ions, such as Cl, HCO₃, and Na, particularly in the kidney. Although the intestinal epithelium also expressed PTH receptors, little was known regarding its mechanism in the regulation of intestinal ion transport. We investigated(More)
Thongon N, Nakkrasae L, Thongbunchoo J, Krishnamra N, Charoenphandhu N. Enhancement of calcium transport in Caco-2 monolayer through PKC -dependent Cav1.3-mediated transcellular and rectifying paracellular pathways by prolactin. Am J Physiol Cell Physiol 296: C1373–C1382, 2009. First published April 1, 2009; doi:10.1152/ajpcell.00053.2009.—Previous(More)