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An energy-conserving pyruvate-to-acetate pathway in Entamoeba histolytica. Pyruvate synthase and a new acetate thiokinase.
Under anaerobic conditions, cells of Entamoeba histolytica grown with bacteria produce H2 and acetate while cells grown axenically produce neither. Aerobically, acetate is produced and O2 is consumedExpand
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Pyrophosphate:D-fructose 6-phosphate 1-phosphotransferase. A new enzyme with the glycolytic function of 6-phosphofructokinase.
Abstract The major enzyme of Entamoeba histolytica which produces fructose 1,6-diphosphate from fructose 6-phosphate utilizes inorganic pyrophosphate as phosphate donor. In its reverse reaction theExpand
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Biochemistry of the dog hookworm. II. Nature and origin of the excreted fatty acids.
Abstract The fermentation of d -glucose-14C by adult Ancylostoma caninum leads to the production of radioactive acetate, propionate, and carbon dioxide. The propionate appears to be a major endExpand
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Antigen presentation by epithelial cells of the rat small intestine. II. Selective induction of suppressor T cells.
Columnar epithelial cells (EC) isolated from the rat small intestine can present protein antigen in vitro, inducing proliferation of primed T cells. This system was used to examine theExpand
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Antigen presentation by epithelial cells of the rat small intestine. I. Kinetics, antigen specificity and blocking by anti-Ia antisera.
Columnar epithelial cells (EC), isolated from the proximal small intestine of the rat, bind ovalbumin (OVA) by a non-specific, cold-inhibitable mechanism and continue to express Ia antigens after 24Expand
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Aerobic and anaerobic metabolism in Entamoeba histolytica.
Abstract Respiration by Entamoeba histolylica is confirmed. A doubling of the rate of oxygen uptake was observed upon the addition of d -glucose to cells in which the glycogen reserve had beenExpand
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A pathway for the interconversion of hexose and pentose in the parasitic amoeba Entamoeba histolytica.
Isotope studies indicate that hexose-to-pentose interconversion by axenic Entamoeba histolytica conserves the C-1 and C-6 hexose carbon atoms. Transketolase was readily identified in amoebalExpand
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Induced division synchrony in Entamoeba histolytica. Effects of hydroxyurea and serum deprivation.
Synchronous growth of axenic Entamoeba histolytica was induced by nutritional deprivation and/or hydroxyurea. Axenic amebae maintained in 2% serum culture medium for 17-25 hours and returned toExpand
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Entamoeba histolytica: membrane fractions.
Abstract A cell fractionation study was made of Entamoeba histolytica, a parasitic amoeba which lacks many typical subcellular organelles. Two membrane fractions were isolated from amoebalExpand
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