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The thiobarbituric acid assay of sialic acids.
- L. Warren
- Chemistry, Medicine
- The Journal of biological chemistry
- 1 August 1959
This chapter discusses the different aspects of thiobarbituric acid assay of sialic acid, which is suitable for measuring the release of bound sialoic acid by sialidase and hydrolysis of sIALic acid-containing material must be carried out for the measurement of total sialsic acids. Expand
Biosynthesis of sialic acids by Neisseria meningitidis.
The over-all biosynthetic reaction for rat liver and bovine submaxillary gland is catalyzed by 3 enzymes: a kinase, a condensing enzyme, and a dephosphorylating enzyme. Expand
The biosynthesis of cytidine 5'-monophospho-n-acetylneuraminic acid by an enzyme from Neisseria meningitidis.
In a preliminary communication, a neK type of reaction carried out by another enzyme of Ncisserio meningitidis, strain 1998, which might be considered the activation of N-acetylneuraminic acid is reported. Expand
Thiobarbituric Acid Spray Reagent for Deoxy Sugars and Sialic Acids
- L. Warren
- 16 April 1960
RECENTLY, new sensitive assays have been reported for deoxy sugars1, 2-keto,3-deoxy sugar acids2–4, and sialic acids5,6. In these assays, the products of periodate oxidation, malonaldehyde from deoxy… Expand
Antibody-dependent cytotoxicity mediated by natural killer cells is enhanced by castanospermine-induced alterations of IgG glycosylation.
Lymphocyte-mediated antibody-dependent cytotoxicity was enhanced significantly, as compared to native (unmodified) IgG-sensitized target cells, when target cells were sensitized with IgG bearing the atypical oligosaccharides induced metabolically by castanospermine, N-methyldeoxynojirimycin or monesin. Expand
Sialic acid in human semen and in the male genital tract.
- L. Warren
- Medicine, Chemistry
- The Journal of clinical investigation
- 1 May 1959
Secretion of lysosomal enzymes by drug-sensitive and multiple drug-resistant cells.
It is proposed that, in a multiple drug-resistant cell such as CEM/VLB100, the presence of P-170 in the plasma membrane may, in some indirect manner, lead to increased exocytosis of lysosomal enzyme, ultimately resulting in a significant depletion of enzyme. Expand
Membranes of animal cells. I. Methods of isolation of the surface membrane
Procedures have been devised for the isolation of the surface membranes of mouse fibroblasts (L cell) and a variety of other cells. The surface membranes are stabilized by various reagents in a… Expand
BIOCHEMICAL AND HISTOCHEMICAL IDENTIFICATION OF SIALIC ACID CONTAINING MUCINS OF RODENT VAGINA AND SALIVARY GLANDS
The mucins elaborated in the mucification reaction of the vagina of the rat and mouse, like salivary mucins, show the histochemical properties of a nonsulfated acid mucopolysaccharide. In the mouse… Expand