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C-terminal half of Salmonella enterica WbaP (RfbP) is the galactosyl-1-phosphate transferase domain catalyzing the first step of O-antigen synthesis
It is proposed that the T block operates prior to the flippase function, probably at the release of undecaprenyl pyrophosphate-linked galactose from WbaP.
Genomic organization of LPS-specific loci.
Lipopolysaccharide genes have many of the characteristics of PAIs but also differ in significant ways; in this these discretionary components do resemble the products of PAI genes.
Sequence of the E. coli O104 antigen gene cluster and identification of O104 specific genes.
Involvement of the galactosyl-1-phosphate transferase encoded by the Salmonella enterica rfbP gene in O-antigen subunit processing
It is shown that rfbP(T) mutants are not blocked in the ligation step as previously believed but in an earlier step, possibly in flipping the O-antigen subunit on undecaprenyl pyrophosphate from the cytoplasmic to periplasmic face of the cytopsized membrane.
Molecular analysis of a Salmonella enterica group E1 rfb gene cluster: O antigen and the genetic basis of the major polymorphism.
Both rfb gene clusters are low in G + C content, indicating that they were transferred from a common ancestral species with low G +C content to S. enterica relatively recently (in the evolutionary sense).
Expression of the O antigen gene cluster is regulated by RfaH through the JUMPstart sequence.
RfaH enhances expression of the 18-kb O antigen gene cluster, with promoter-distal genes affected more dramatically, and it was shown that the JUMPstart sequence was required for RfaH function.
Localizing the replication origin region on the physical map of the Mycoplasma capricolum genome
Four lines of evidence argue that the replication origin of the Mycoplasma capricolum genome lies within the 46-kb BamHI fragment bordered by two BamHI sites of the total of nine BamHI sites that…
Bacterial expression of the major antigenic regions of porcine rotavirus VP7 induces a neutralizing immune response in mice.
Recombination and DNA replication in Bacillus subtilis transformation.
Physical mapping of the Mycoplasma capricolum genome.
A physical map of Mycoplasma capricolum ATCC 27343 genome was constructed, based on estimation of the restriction fragment sizes by pulse-field electrophoresis, which established the genome size at 1155.5 kb and assigned 26 cleavage sites for 7 endonucleases.