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Estimating the probability of identity among genotypes in natural populations: cautions and guidelines
An equation for P(ID) between sibs is introduced that provides an estimator that can be used as a conservative upper bound for the probability of observing identical multilocus genotypes between two individuals sampled from a population.
Reliable genotyping of samples with very low DNA quantities using PCR.
An experimental procedure using PCR that provides a reliable genotype at a microsatellite locus using only a few picograms of template DNA is identified and should be systematically used when genotyping nuclear loci of ancient or forensic samples, museum specimens and hair or feces of free ranging animals.
Landscape genetics: where are we now?
- A. Storfer, M. Murphy, S. Spear, R. Holderegger, L. Waits
- Biology, MedicineMolecular ecology
- 1 September 2010
Highlights gaps in knowledge and methodology are highlighted, providing guidelines to authors and reviewers of landscape genetics studies, and suggesting promising future directions of inquiry are suggested.
Noninvasive genetic tracking of the endangered Pyrenean brown bear population
Comprehensive knowledge of endangered small populations of mammals can be obtained using noninvasive genetic sampling and it is suggested that managers consider population augmentation using only females to preserve the remaining Pyrenean gene pool and increase genetic diversity.
An empirical evaluation of genetic distance statistics using microsatellite data from bear (Ursidae) populations.
- D. Paetkau, L. Waits, P. Clarkson, L. Craighead, C. Strobeck
- Biology, MedicineGenetics
- 1 December 1997
A large microsatellite data set from three species of bear was used to empirically test the performance of six genetic distance measures in resolving relationships at a variety of scales, finding that linearity was lost for each of the statistics.
A new method for estimating the size of small populations from genetic mark–recapture data
It is concluded that capwire provides an improved way to estimate N for some DNA‐based data sets, and is compared to commonly used rarefaction methods and to two heterogeneity estimators in program capture: Mh‐Chao and Mh-jackknife.
Statistical approaches in landscape genetics: an evaluation of methods for linking landscape and genetic data
Results suggest that some of the most commonly applied techniques in landscape genetics have high type-1 error rates, and that multivariate, non-linear methods are better suited for landscape genetic data analysis.
NONINVASIVE GENETIC SAMPLING TOOLS FOR WILDLIFE BIOLOGISTS: A REVIEW OF APPLICATIONS AND RECOMMENDATIONS FOR ACCURATE DATA COLLECTION
The recent advancements and techniques used for identifying species, individuals, and gender are reviewed and recommendations for laboratory- and field-based methods to improve the reliability and accuracy of data collected from noninvasive genetic samples are provided.
Estimating occupancy and abundance of stream amphibians using environmental DNA from filtered water samples
Environmental DNA (eDNA) methods for detecting aquatic species are advancing rapidly, but with little evaluation of field protocols or precision of resulting estimates. We compared sampling results…
Molecular Detection of Vertebrates in Stream Water: A Demonstration Using Rocky Mountain Tailed Frogs and Idaho Giant Salamanders
The utility of eDNA techniques for detecting aquatic vertebrates in stream systems has been demonstrated across the majority of freshwater systems, setting the stage for an innovative transformation in approaches for aquatic research.