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DNA binding and cleavage by the periplasmic nuclease Vvn: a novel structure with a known active site
The Vibrio vulnificus nuclease, Vvn, is a non‐specific periplasmic nuclease capable of digesting DNA and RNA. The crystal structure of Vvn and that of Vvn mutant H80A in complex with DNA wereExpand
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Structural studies of the pigeon cytosolic NADP+‐dependent malic enzyme
  • Z. Yang, H. Zhang, +6 authors L. Tong
  • Biology, Medicine
  • Protein science : a publication of the Protein…
  • 1 February 2002
Malic enzymes are widely distributed in nature, and have important biological functions. They catalyze the oxidative decarboxylation of malate to produce pyruvate and CO2 in the presence of divalentExpand
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Metal ions and phosphate binding in the H‐N‐H motif: Crystal structures of the nuclease domain of ColE7/Im7 in complex with a phosphate ion and different divalent metal ions
  • M. Sui, L. Tsai, +4 authors H. Yuan
  • Chemistry, Medicine
  • Protein science : a publication of the Protein…
  • 1 December 2002
H‐N‐H is a motif found in the nuclease domain of a subfamily of bacteria toxins, including colicin E7, that are capable of cleaving DNA nonspecifically. This H‐N‐H motif has also been identified in aExpand
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Lysine residues 162 and 340 are involved in the catalysis and coenzyme binding of NADP(+)-dependent malic enzyme from pigeon.
Alanine-scanning site-directed mutagenesis was carried out on all conserved lysine residues of pigeon cytosolic NADP(+)-dependent malic enzyme. Only two mutant enzymes, K162A and K340A, showedExpand
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Directed Mutagenesis of Specific Active Site Residues onFibrobacter succinogenes1,3–1,4-β-d-Glucanase Significantly Affects Catalysis and Enzyme Structural Stability*
The functional and structural significance of amino acid residues Met39, Glu56, Asp58, Glu60, and Gly63 ofFibrobacter succinogenes1,3–1,4-β-d-glucanase was explored by the approach of site-directedExpand
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Mutagenesis of Trp(54) and Trp(203) residues on Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase significantly affects catalytic activities of the enzyme.
The possible structural and catalytic functions of the nine tryptophan amino acid residues, including Trp(54), Trp(105), Trp(112), Trp(141), Trp(148), Trp(165), Trp(186), Trp(198), and Trp(203) inExpand
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Crystal structure of truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase in complex with beta-1,3-1,4-cellotriose.
Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase (Fsbeta-glucanase) catalyzes the specific hydrolysis of beta-1,4 glycosidic bonds adjacent to beta-1,3 linkages in beta-D-glucans or lichenan. ThisExpand
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Crystal structure of a natural circularly permuted jellyroll protein: 1,3-1,4-beta-D-glucanase from Fibrobacter succinogenes.
The 1,3-1,4-beta-D-glucanase from Fibrobacter succinogenes (Fsbeta-glucanase) is classified as one of the family 16 glycosyl hydrolases. It hydrolyzes the glycosidic bond in the mixed-linked glucansExpand
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Improving nanowire sensing capability by electrical field alignment of surface probing molecules.
We argue that the structure ordering of self-assembled probing molecular monolayers is essential for the reliability and sensitivity of nanowire-based field-effect sensors because it can promote theExpand
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Control and Detection of Organosilane Polarization on Nanowire Field-Effect Transistors
We demonstrated control and detection of UV-induced 3-aminopropyltriethoxysilane (APTES) polarization using silicon nanowire field-effect transistors made by top-down lithograph technology. TheExpand
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