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Combinatorial delivery of small interfering RNAs reduces RNAi efficacy by selective incorporation into RISC
TLDR
It is shown that both synthetic siRNAs and expressed shRNAs compete against each other and with the endogenous microRNAs for transport and for incorporation into the RNA induced silencing complex (RISC).
Minibody: A novel engineered anti-carcinoembryonic antigen antibody fragment (single-chain Fv-CH3) which exhibits rapid, high-level targeting of xenografts.
TLDR
Purified minibodies retained high affinity for CEA (KA, 2 x 10(9) M(-1)) and demonstrated bivalent binding to antigen and tumor targeting properties were evaluated in vivo using athymic mice bearing LS174T human colon carcinoma xenografts.
Molecular cloning of a cDNA coding biliary glycoprotein I: primary structure of a glycoprotein immunologically crossreactive with carcinoembryonic antigen.
TLDR
It is proposed that BGP I diverged from NCA by acquiring an immunoglobulin-like domain substantially different from the domains found in NCA or CEA and also a new cytoplasmic domain.
Cloning of the genes for T84.66, an antibody that has a high specificity and affinity for carcinoembryonic antigen, and expression of chimeric human/mouse T84.66 genes in myeloma and Chinese hamster
TLDR
The cloning and sequencing of the genes coding for monoclonal antibody T84.66 and the amino acid sequence of the variable regions for the heavy and light chains were reported and the chimeric antibodies obtained exhibited the same specificity and affinity for CEA as that of the T 84.66 immunoglobulin produced by the murine hybridoma cell line.
Isolation of a cDNA clone for human X-linked 3-phosphoglycerate kinase by use of a mixture of synthetic oligodeoxyribonucleotides as a detection probe.
TLDR
A cDNA clone encoding most of human X-linked 3-phosphoglycerate kinase (PGK) was obtained and DNA sequence analysis has established that the 1.2-kilobase-pair fragment encodes PGK from amino acid 121 through the COOH terminus.
Real-time PCR assay for quantitative mismatch detection.
TLDR
Because the initial amplification is separate from real-time detection, conditions can be independently optimized for each step, making the assay particularly suitable for the detection of allele-specific expression in single cells.
Molecular cloning of a gene belonging to the carcinoembryonic antigen gene family and discussion of a domain model.
TLDR
It is likely that this gene family evolved from a common ancestor shared with neural cell adhesion molecule and alpha 1 B-glycoprotein and is perhaps a family within the immunoglobulin superfamily.
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