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Determination of tyrosine exposure in proteins by second-derivative spectroscopy.
TLDR
The mutual interference between the second-derivative bands of tyrosine and tryptophan in proteins has been evaluated in terms of the ratio r and the results obtained on purified proteins have been found consistent with the available X-ray information and with the existing solvent perturbation data. Expand
A glycoprotein inhibitor of pectin methylesterase in kiwi fruit (Actinidia chinensis).
TLDR
Kiwi inhibitor appears to be specific for pectin methylesterase, inasmuch as it was found to be ineffective against other polysaccharide-degrading enzymes, such as polygalacturonase and amylase. Expand
Pectin methylesterase inhibitor.
TLDR
A large protein family including PMEI, invertase inhibitors and related proteins of unknown function has been identified on the basis of amino acid sequence similarity and Cys residues conservation. Expand
A glycoprotein inhibitor of pectin methylesterase in kiwi fruit. Purification by affinity chromatography and evidence of a ripening-related precursor.
TLDR
The results showed that the inhibitor is present in the unripe fruit as an inactive precursor with a higher molecular mass and is transformed into the active protein, most likely by proteinase action, during the course of the ripening process. Expand
Kiwi protein inhibitor of pectin methylesterase
TLDR
The comparison of the sequence of these inhibitors confirms the existence of a novel class of proteins with moderate but significant sequence conservation, comprising plant proteins acting as inhibitors of sugar metabolism enzymes, and probably involved in various steps of plant development. Expand
Tomato pectin methylesterase: Modeling, fluorescence, and inhibitor interaction studies—comparison with the bacterial (Erwinia chrysanthemi) enzyme
TLDR
Fluorescence quenching experiments on tomato PME and on PME‐PMEI complex suggest that tryptophanyl residues present in the active site region are involved in the interaction and that the inhibitor interacts with plant PME at the level of the activeSite, supporting the hypothesis that Asp132 acts as acid/base in the catalytic cycle. Expand
Kiwi protein inhibitor of pectin methylesterase amino-acid sequence and structural importance of two disulfide bridges.
TLDR
The comparison of the sequence of pectin methylesterase inhibitors confirms the existence of a novel class of proteins with moderate but significant sequence conservation, comprising plant proteins acting as inhibitors of sugar metabolism enzymes, and probably involved in various steps of plant development. Expand
Determination of estragole, safrole and eugenol methyl ether in food products
Abstract A rapid and effective procedure for the extraction and determination of 4-allylanisole (estragole), 4-allyl-1,3-benzodioxole (safrole) and 4-allyl-1,2-dimetoxybenzene (eugenol methyl ether)Expand
SIRT1 and SIRT6 Signaling Pathways in Cardiovascular Disease Protection.
TLDR
A comprehensive overview of recent developments on the molecular signaling pathways controlled by SIRT1 and SIRT6, two post-translational modifiers proven to be valuable tools to dampen inflammation and oxidative stress at the cardiovascular level is provided. Expand
An uncommon redox behavior sheds light on the cellular antioxidant properties of ergothioneine.
TLDR
Treatment of human neutrophils with phorbol 12-myristate 13-acetate in the presence of ESH leads to a remarkable production of the sulfonated form (ESO3H), a compound never described before, and hercynine (EH), the desulfurated form of E SH, suggesting a mechanism through which ESH performs its antioxidant action in cells. Expand
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