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The 193-Kd Vault Protein, Vparp, Is a Novel Poly(Adp-Ribose) Polymerase
TLDR
The 193-kD vault protein is identified by its interaction with the MVP in a yeast two-hybrid screen and confirmed its identity by peptide sequence analysis, and it is shown that one substrate for this vault-associated PARP activity is the MVP. Expand
Reassessment of Exosome Composition
TLDR
High-resolution density gradient fractionation and direct immunoaffinity capture are employed to precisely characterize the RNA, DNA, and protein constituents of exosomes and other non-vesicle material and show that small extracellular vesicles are not vehicles of active DNA release. Expand
Vaults Are Up-regulated in Multidrug-resistant Cancer Cell Lines*
TLDR
The observation that vault synthesis is linked directly to multidrug resistance supports a direct role for vaults in drug resistance. Expand
Vaults and Telomerase Share a Common Subunit, TEP1*
TLDR
It is shown that while TEP1 is a component of the vault particle, vaults have no detectable telomerase activity, suggesting that TEP 1 may play a common role in some aspect of ribonucleoprotein structure, function, or assembly. Expand
Vault ribonucleoprotein particles from rat and bullfrog contain a related small RNA that is transcribed by RNA polymerase III.
TLDR
The rat v RNA gene was efficiently transcribed in vitro, producing a single transcript of about 140 bases, and unlike most RNA polymerase III genes, the rat vRNA is present as a single copy gene and has a distinct tissue-specific expression pattern. Expand
Two species of lysosomal organelles in cultured human fibroblasts
TLDR
Cultured diploid human skin fibroblasts were fractionated by a procedure that maximizes recovery of particles containing acid hydrolases to suggest that the dense and buoyant lysosomal organelles originate primarily from residual bodies and the GERL network, respectively. Expand
Novel CCL21-Vault Nanocapsule Intratumoral Delivery Inhibits Lung Cancer Growth
TLDR
This study demonstrates that the vault nanocapsule can efficiently deliver CCL21 to sustain antitumor activity and inhibit lung cancer growth and can serve as an “off the shelf” approach to deliver antitUMor cytokines to treat a broad range of malignancies. Expand
Sizing large proteins and protein complexes by electrospray ionization mass spectrometry and ion mobility
TLDR
There is a strong correlation between the electrophoretic mobility diameter determined from GEMMA analysis and the molecular mass for protein complexes up to 12 MDa, including the 93 kDa enolase dimer, the 480 kDa ferritin 24-mer complex, and the 9 MDa MVP-vault assembly. Expand
The Telomerase/Vault-Associated Protein Tep1 Is Required for Vault RNA Stability and Its Association with the Vault Particle
TLDR
A novel role for TEP1 in vivo as an integral vault protein important for the stabilization and recruitment of the vault RNA to the vault particle is uncovered. Expand
A Vault Nanoparticle Vaccine Induces Protective Mucosal Immunity
TLDR
Vault nanoparticles containing immunogenic proteins delivered to the respiratory tract by the i.n. route can act as “smart adjuvants” for inducing protective immunity at distant mucosal surfaces while avoiding destructive inflammation. Expand
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