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Structural model for family 32 of glycosyl‐hydrolase enzymes
A structural model is presented for family 32 of the glycosyl‐hydrolase enzymes based on the beta‐propeller fold derived from the common prediction of two different threading methods, TOPITS and THREADER, and proposes the participation of one more conserved residue (Asp 162) in the catalytic mechanism.
Purification and characterization of the invertase from Schizosaccharomyces pombe. A comparative analysis with the invertase from Saccharomyces cerevisiae.
Invertase (EC was purified to homogeneity from exponentially growing cells of Schizosaccharomyces pombe fully de-repressed for synthesis of the enzyme, and was shown to be a
Cloning and sequence analysis of the gene encoding invertase (INV1) from the yeast Candida utilis
The gene INV1 encoding invertase from the yeast Candida utilis has been cloned using a homologous PCR hybridization probe, amplified with two sets of degenerate primers designed considering sequence
Characterization of recombinant invertase expressed in methylotrophic yeasts
Characterization of the invertase expressed in the methylotrophic yeasts Hansenula polymorpha and Pichia pastoris in terms of enzyme conformational stability and structural behaviour induced by temperature as a function of pH suggests that irreversibility could be associated with slow refolding kinetics at high protein concentration.
Invertase secretion in Hansenula polymorpha under the AOX1 promoter from Pichia pastoris
A DNA fragment containing a transcription regulating region of the alcohol oxidase (AOX1) gene from the methylotrophic yeast Pichia pastoris was used in the construction of a vector for the
Uptake of sucrose by Saccharomyces cerevisiae.
Development of an integrative DNA transformation system for the yeast Candida utilis.
The development of an auxotrophic transformation system for the food yeast Candida utilis by using both, LiAc and electroporation methods to direct insertions at the ura3 locus through homologous recombination is reported.
Role of glutamine aminotransferase in glutamine catabolism by Saccharomyces cerevisiae under microaerophilic conditions.
There are at least two different glutamine aminotransferase activities that are differentiated genetically, by their substrate specificity (pyruvate and glyoxylate dependence), and their different modes of regulation.
Temperature-sensitive forms of large and small invertase in a mutant derived from a SUC1 strain of Saccharomyces cerevisiae.
Findings are evidence that SUC1 is a structural gene for invertase and that both large and small forms are encoded by a single gene.