Biochemical Characterization of Individual Human Glycosylated pro-Insulin-like Growth Factor (IGF)-II and big-IGF-II Isoforms Associated with Cancer
- S. Greenall, J. Bentley, T. Adams
- Biology, MedicineThe Journal of Biological Chemistry
- 19 November 2012
IGF-II isoforms possess unique biological properties that may enable them to escape normal sequestration avenues and remain bioavailable in vivo to sustain oncogenic signaling.
Single-chain Fv fragments of anti-neuraminidase antibody NC10 containing five- and ten-residue linkers form dimers and with zero-residue linker a trimer.
BIAcore biosensor experiments showed that when the scFvs were used as the analyte, the dimeric and trimeric scFVS showed an apparent increase in binding affinity due to the avidity of binding the multivalent scFv.
Kinetic screening of antibody-Im7 conjugates by capture on a colicin E7 DNase domain using optical biosensors.
Structural studies of the tethered N‐terminus of the Alzheimer's disease amyloid‐β peptide
This novel structure has the potential to provide a foundation for investigating the effect of metal ion binding to Aβ and illustrates a potential target for the development of future Alzheimer's disease therapeutics aimed at stabilizing the N‐terminal monomer structure, in particular residues His13 and His14.
Progressive myoclonus epilepsy with Lafora inclusion bodies. II. Studies of ultrastructure.
The ultrastructure of the inclusion bodies found in cerebral cortical biopsy specimens from two patients with Lafora's disease are described, and the ultrast structure of liver and gastrocnemius muscle are briefly described.
ScFv multimers of the anti-neuraminidase antibody NC10: shortening of the linker in single-chain Fv fragment assembled in V(L) to V(H) orientation drives the formation of dimers, trimers, tetramers…
- O. Dolezal, L. Pearce, L. Lawrence, A. Mccoy, P. Hudson, A. Kortt
- Biology, ChemistryProtein Engineering
- 1 August 2000
Analysis of synthetic genes encoding single-chain variable fragments of NC10 anti-neuraminidase antibody showed that as the linker length between V(L) and V(H) was reduced, different patterns of oligomerization were observed, and the transition between dimers and higher order oligomers was not as distinct as for V (H)-V(L).
CD52 glycan binds the proinflammatory B box of HMGB1 to engage the Siglec-10 receptor and suppress human T cell function
- E. Bandala-Sanchez, Naiara G Bediaga, L. Harrison
- BiologyProceedings of the National Academy of Sciences
- 11 July 2018
Significance Inflammation is a protective response of the body’s immune system against harmful stimuli such as pathogenic microorganisms, toxins, or damaged cells. However, if excessive or prolonged,…
Preclinical development of a molecular clamp‐stabilised subunit vaccine for severe acute respiratory syndrome coronavirus 2
- D. Watterson, D. Wijesundara, K. Chappell
- Biology, MedicineClinical & Translational Immunology
- 1 January 2021
Rational antigen design through to manufacturability and vaccine efficacy of a prefusion‐stabilised spike (S) protein, Sclamp, in combination with the licensed adjuvant MF59 ‘MF59C.1’ (Seqirus, Parkville, Australia).
FAMILIAL HYPOKALEMIC PERIODIC PARALYSIS WITH PERMANENT MYOPATHY A CLINICAL AND ULTRASTBCCTURAL STUDY
A patient with familial hypokalemic periodic paralysis associated with permanent weakness and wasting of the limb musculature proximally is evaluated, and some of the clinical and ultrastructural findings differ from those previously recorded.
Familial spinocerebellar degeneration, hemolytic anemia, and glutathione deficiency.
- F. Richards, M. Cooper, L. Pearce, R. Cowan, C. Spurr
- MedicineArchives of internal medicine
- 1 September 1974
Preliminary studies indicate that this may be the only reported family with GSH deficiency due to γglutamyl-cysteine synthetase deficiency, and patients with autosomal recessive forms of spinocerebellar degeneration should be examined for disorders of GSH synthesis.