L. P. Cooper
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The in vitro assembly of the EcoKI type I DNA restriction/modification enzyme and its in vivo implications.
Type I DNA restriction/modification enzymes protect the bacterial cell from viral infection by cleaving foreign DNA which lacks N6-adenine methylation within a target sequence and maintaining the… Expand
Purification and characterization of the methyltransferase from the type 1 restriction and modification system of Escherichia coli K12.
The DNA methyltransferase component of the type I restriction and modification enzyme of Escherichia coli K12 has been purified. The active component, a trimer of molecular mass 170 kDa consisting of… Expand
EcoKI with an amino acid substitution in any one of seven DEAD-box motifs has impaired ATPase and endonuclease activities.
- G. P. Davies, L. Powell, J. Webb, L. P. Cooper, N. Murray
- Biology, Medicine
- Nucleic acids research
- 1 November 1998
For type I restriction systems, recently determined nucleotide sequences predict conserved amino acids in the subunit that is essential for restriction but not modification (HsdR). The conserved… Expand
The domains of a type I DNA methyltransferase. Interactions and role in recognition of DNA methylation.
The DNA methyltransferases of type I restriction-modification systems are trimeric enzymes composed of one DNA specificity (S) subunit and two modification (M) subunits. The S subunit contains two… Expand
Assembly of the multifunctional EcoKI DNA restriction enzyme in vitro
Publisher Summary The archetypal type I system is that of Escherichia coli K12, Eco Kl. This enzyme comprises three different subunits, the specificity (S) subunit that recognizes the DNA sequence… Expand
Purification and Characterization of the ~ ethyItransferase from the Type 1 Restriction and Modification System of Escherichia coli K 12 *
Deep Space Network information system architecture study