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The in vitro assembly of the EcoKI type I DNA restriction/modification enzyme and its in vivo implications.
Type I DNA restriction/modification enzymes protect the bacterial cell from viral infection by cleaving foreign DNA which lacks N6-adenine methylation within a target sequence and maintaining theExpand
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Purification and characterization of the methyltransferase from the type 1 restriction and modification system of Escherichia coli K12.
The DNA methyltransferase component of the type I restriction and modification enzyme of Escherichia coli K12 has been purified. The active component, a trimer of molecular mass 170 kDa consisting ofExpand
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EcoKI with an amino acid substitution in any one of seven DEAD-box motifs has impaired ATPase and endonuclease activities.
For type I restriction systems, recently determined nucleotide sequences predict conserved amino acids in the subunit that is essential for restriction but not modification (HsdR). The conservedExpand
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The domains of a type I DNA methyltransferase. Interactions and role in recognition of DNA methylation.
The DNA methyltransferases of type I restriction-modification systems are trimeric enzymes composed of one DNA specificity (S) subunit and two modification (M) subunits. The S subunit contains twoExpand
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Assembly of the multifunctional EcoKI DNA restriction enzyme in vitro
Publisher Summary The archetypal type I system is that of Escherichia coli K12, Eco Kl. This enzyme comprises three different subunits, the specificity (S) subunit that recognizes the DNA sequenceExpand
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Vehicle/engine integration
Deep Space Network information system architecture study