The complete nucleotide sequence of the bacteriophage VH ML is determined and a hypothesis for the virulence conversion caused by VHML infection of Vibrio harveyi is established.Expand
Primers designed to detect epizootic haematopoietic necrosis virus and a PCR and dot-blot system were used successfully in screening for the presence of BIV nucleic acid in digested formalin-fixed, paraffin-embedded amphibian tissues, and PCR could detect BIV in amphibians long after BIV challenge, and in frogs which appeared healthy.Expand
The cause of ongoing mortality in barramundi Lates calcarifer (Bloch) in seawater culture was identified as Streptococcus iniae by biochemical and physiological tests, and the organism was easily isolated from the brain of both moribund and healthy fish, indicating that barramsundi can carry the bacterium asymptomatically.Expand
Two luminous strains of Vibrio harveyi, previously demonstrated to be virulent to Penaeus monodon larvae were shown to produce proteinaceous exotoxins capable of causing mortality in mice and P.… Expand
Increased levels of nitrate occur in natural waters due to pollution, and in aquaculture systems from nitrification and addition of microalgal cultures for feeding. Static bioassays showed that… Expand
The longest lasting, non- proliferating, but viable, cell cultures were those of subcutis/epidermis and heart cells which remained viable for 240 and 307 days respectively, and only cell cultures from multiple prawns achieved 100% confluency in 25 cm(2) plastic culture flasks.Expand
VHML is a temperate bacteriophage that has a narrow host range and shows an apparent preference for V. harveyi above other vibrios and other genera and the conventional methods for phage concentration and extraction of nucleic acids from phage particles were not efficient.Expand
Results indicated that IHHNV has been present in Australia for a long (geological and contemporary) time and that the virus is endemic in penaeid prawns in the Australian environment.Expand
This agar displays great potential as a primary isolation medium and offers significant advantages over thiosulfate-citrate-bile salts-sucrose agar as a medium for differentiating V. harveyi from other marine and estuarine Vibrio species.Expand
In the future, it is expected that the world shrimp industry will have ready access to a variety of domesticated, genetically improved shrimp stocks free of all significant pathogens.Expand