• Publications
  • Influence
COPII: A membrane coat formed by Sec proteins that drive vesicle budding from the endoplasmic reticulum
In vitro synthesis of endoplasmic reticulum-derived transport vesicles has been reconstituted with washed membranes and three soluble proteins (Sar1p, Sec13p complex, and Sec23p complex). VesicleExpand
  • 1,219
  • 117
Bi-directional protein transport between the ER and Golgi.
The endoplasmic reticulum (ER) and the Golgi comprise the first two steps in protein secretion. Vesicular carriers mediate a continuous flux of proteins and lipids between these compartments,Expand
  • 802
  • 57
  • PDF
COPII-Coated Vesicle Formation Reconstituted with Purified Coat Proteins and Chemically Defined Liposomes
COPII vesicle formation requires only three coat assembly subunits: Sar1p, Sec13/31p, and Sec23/24p. PI 4-phosphate or PI 4,5-bisphosphate is required for the binding of these proteins to liposomes.Expand
  • 599
  • 51
Lipotoxic heart disease in obese rats: implications for human obesity.
To determine the mechanism of the cardiac dilatation and reduced contractility of obese Zucker Diabetic Fatty rats, myocardial triacylglycerol (TG) was assayed chemically and morphologically. TG wasExpand
  • 1,187
  • 49
  • PDF
Identification of a fibroblast-derived epithelial morphogen as hepatocyte growth factor
We have previously shown that Madin-Darby canine kidney (MDCK) epithelial cells grown in collagen gels in the presence of fibroblasts or fibroblast-conditioned medium (CM) form branching tubules,Expand
  • 1,120
  • 36
Coat Proteins and Vesicle Budding
The trafficking of proteins within eukaryotic cells is achieved by the capture of cargo and targeting molecules into vesicles that bud from a donor membrane and deliver their contents to a receivingExpand
  • 939
  • 34
Multiple Cargo Binding Sites on the COPII Subunit Sec24p Ensure Capture of Diverse Membrane Proteins into Transport Vesicles
We have characterized the mechanisms of cargo selection into ER-derived vesicles by the COPII subunit Sec24p. We identified a site on Sec24p that recognizes the v-SNARE Bet1p and show that packagingExpand
  • 455
  • 34
  • PDF
Dynamics of the COPII coat with GTP and stable analogues
We have developed an assay to monitor the assembly of the COPII coat onto liposomes in real time. We show that with Sar1pGTP bound to liposomes, a single round of assembly and disassembly of theExpand
  • 292
  • 33
  • PDF
Defective prohormone processing and altered pancreatic islet morphology in mice lacking active SPC2.
The prohormone convertase SPC2 (PC2) participates in the processing of proinsulin, proglucagon, and a variety of other neuroendocrine precursors, acting either alone or in conjunction with theExpand
  • 401
  • 29
  • PDF
A major transmembrane protein of Golgi-derived COPI-coated vesicles involved in coatomer binding
Formation of non-clathrin-coated vesicles requires the recruitment of several cytosolic factors to the Golgi membrane. To identify membrane proteins involved in this budding process, a highlyExpand
  • 228
  • 29
  • PDF