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Fuel ethanol production from lignocellulose: a challenge for metabolic engineering and process integration
Improvement of the fermentation process is just one of several factor that needs to be fully optimized and integrated to generate a competitive lignocellulose ethanol plant.
Fermentation of lignocellulosic hydrolysates for ethanol production.
Ethanol production from lignocellulosic hydrolysates in an economically feasible process requires microorganisms that produce ethanol with a high yield from all sugars present (hexoses as well as
Increasing NADH oxidation reduces overflow metabolism in Saccharomyces cerevisiae
Results indicate that NADH oxidase localizes in the cytosol, whereas alternative oxidase is directed to the mitochondria, which indicates that the Crabtree effect is due to limited respiratory capacity or is caused by glucose-mediated repression of respiration.
Metabolic Engineering of Saccharomyces cerevisiae
Strategies and concepts for metabolic engineering using Saccharomyces cerevisiae illustrate all the categories of this multidisciplinary field: extension of substrate range, improvements of producitivity and yield, elimination of byproduct formation, improvement of process performance, and extensions of cellular properties.
Lignocellulosic ethanol production at high-gravity: challenges and perspectives.
This review focuses on challenges and technological efforts in processing at high-gravity conditions and how these conditions influence the physiology and metabolism of fermenting microorganisms, the action of enzymes, and other process-related factors.
The influence of HMF and furfural on redox-balance and energy-state of xylose-utilizing Saccharomyces cerevisiae
The redox metabolism was severely affected by HMF and furfural while the effects on energy metabolism were less evident, suggesting that engineering of the redox system represents a possible strategy to develop more robust strains for bioethanol production.
On-line and in situ monitoring of biomass in submerged cultivations
Biomass is one of the most important variables in submerged-cultivation processes and, in recent years, many different sensors have been introduced for on-line or in situ monitoring of this variable.
Characterization and kinetic analysis of a thermostable GH3 β-glucosidase from Penicillium brasilianum
A novel assay was developed to characterize this glucose inhibition on cellobiose hydrolysis of GH3 β-glucosidase, and it was successfully expressed in Aspergillus oryzae.
Improvement of Galactose Uptake in Saccharomyces cerevisiae through Overexpression of Phosphoglucomutase: Example of Transcript Analysis as a Tool in Inverse Metabolic Engineering
It is concluded that phosphoglucomutase plays a key role in controlling the flux through the Leloir pathway, probably due to increased conversion of glucose-1-phosphate to glucose-6-ph phosphate in the strain construct overexpressing PGM2.
Comparison of SHF and SSF processes from steam‐exploded wheat straw for ethanol production by xylose‐fermenting and robust glucose‐fermenting Saccharomyces cerevisiae strains
Ethanol concentrations obtained in SSF processes were in all cases higher than those from SHF at the same conditions and final ethanol concentration was improved in all tests due to the increase of potential fermentable sugars in the fermentation broth.