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Deletion of the Escherichia coli pseudouridine synthase gene truB blocks formation of pseudouridine 55 in tRNA in vivo, does not affect exponential growth, but confers a strong selective disadvantage
TLDR
It is shown that TruB is the only protein in E. coli able to make psi55 in vivo, and all growth effects could be reversed by transformation with a plasmid carrying a truB gene coding for a D48C mutation in TruB. Expand
Saccharofermentans acetigenes gen. nov., sp. nov., an anaerobic bacterium isolated from sludge treating brewery wastewater.
TLDR
On the basis of evidence from this polyphasic study, strain P6(T) is considered to represent a novel species of a new genus, for which the name Saccharofermentans acetigenes gen. nov., sp. Expand
Functional Effect of Deletion and Mutation of theEscherichia coli Ribosomal RNA and tRNA Pseudouridine Synthase RluA*
TLDR
Plasmid rescue of bothrluA − strains using an rluA gene carrying asparagine or threonine replacements for the highly conserved aspartate 64 demonstrated that neither mutant could form 23 S RNA pseudouridine 746 or tRNA pseudOURidine 32 in vivo, showing that this conservedAspartate is essential for enzyme-catalyzed formation of both pseudouridines. Expand
Tepidimicrobium xylanilyticum sp. nov., an anaerobic xylanolytic bacterium, and emended description of the genus Tepidimicrobium.
TLDR
It is suggested that strain PML14(T), isolated from the sludge of a thermophilic anaerobic digester treating municipal solid waste and sewage in Beijing, China, represents a novel species of the genus Tepidimicrobium, for which the name Tepidonium xylanilyticum sp. Expand
16S ribosomal RNA pseudouridine synthase RsuA of Escherichia coli: deletion, mutation of the conserved Asp102 residue, and sequence comparison among all other pseudouridine synthases.
TLDR
Induction of wild-type and the two mutant synthases in strain BL21/DE3 from genes in pET15b yielded a strong overexpression of all three proteins in approximately equal amounts showing that the mutations did not affect production of the protein in vivo and thus that the lack of activity was not due to a failure to produce a gene product. Expand
Proteiniborus ethanoligenes gen. nov., sp. nov., an anaerobic protein-utilizing bacterium.
TLDR
On the basis of polyphasic evidence from this study, strain GW(T) represents a new genus and novel species, for which the name Proteiniborus ethanoligenes gen. nov., sp. Expand
Aeromicrobium camelliae sp. nov., isolated from Pu'er tea.
Paenibacillus yunnanensis sp. nov., isolated from Pu'er tea.
TLDR
Strain YN2T could be differentiated from recognized species of the genus Paenibacillus based on phenotypic characteristics, chemotaxonomic differences, phylogenetic analysis and DNA–DNA hybridization data. Expand
Bacillus camelliae sp. nov., isolated from Pu'er tea.
TLDR
On the basis of the presented polyphasic evidence, strain 7578-1T is considered to represent a novel species of the genus Bacillus, for which the name Bacillus camelliae sp. Expand
Pullulanibacillus pueri sp. nov., isolated from Pu'er tea.
TLDR
Strain YN3(T) could be differentiated from recognized species of the genus Pullulanibacillus based on phenotypic characteristics, chemotaxonomic differences, phylogenetic analysis and DNA-DNA hybridization data. Expand
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