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Taura syndrome virus (TSV) in Thailand and its relationship to TSV in China and the Americas.
- L. Nielsen, Wiwat Sang-oum, S. Cheevadhanarak, T. Flegel
- Biology, Medicine
- Diseases of aquatic organisms
- 28 February 2005
To determine the origin of the TSV in Thailand, the viral coat protein gene VP1 was amplified by RT-PCR from several shrimp specimens found positive for TSV by RT, which revealed that the Thai, Burmese and Chinese TSV types formed aClade distinct from a clade of TSVtypes from the Americas. Expand
Detection and subtyping (H5 and H7) of avian type A influenza virus by reverse transcription-PCR and PCR-ELISA
This is the first demonstration of RT-PCR detection on a panel of H7 strains using only one primer set and the H7 origin and the pathogenic potential defined by the presence or absence of basic amino acids at the cleavage site can be determined by sequencing of the PCR product. Expand
Genetic diversity in the yellow head nidovirus complex
The data support previous evidence that ORF4 is a non-functional gene under construction or deconstruction and contain predicted 3′-terminal hairpin-loop structures that are preserved in all genotypes by compensatory nucleotide substitutions, suggesting a role in polymerase recognition for minus-strand RNA synthesis. Expand
Presence of multiple viruses in non-diseased, cultivated shrimp at harvest
Histological examinations were carried out with 400 cultivated black tiger shrimp from 12 commercial rearing ponds from three different areas in Thailand over a period of 3 years, finding that HPV infection was correlated with more severe stunting than MBV. Expand
Comparison of PCR testing methods for white spot syndrome virus (WSSV) infections in penaeid shrimp
The results confirmed the validity of PCR-based methods in Thailand for detection of white spot syndrome virus (WSSV) infection in penaeid shrimp post larvae (PL) before they are stocked in rearing ponds. Expand
Comparison of penaeid shrimp and insect parvoviruses suggests that viral transfers may occur between two distantly related arthropod groups.
- Songsak Roekring, L. Nielsen, L. Owens, S. Pattanakitsakul, P. Malasit, T. Flegel
- Biology, Medicine
- Virus research
- 1 July 2002
The DNA and putative amino acid sequences of representative insect and shrimp parvoviruses (subfamily Densovirinae) were analyzed using computer programs and no significant DNA homology was found except for 77% DNA identity between HPVmon and HPVchin. Expand
investigation ofthetissue distribution ofmannan-binding lectin innon-infected andvirus-infected chickens
Findings indicate the liver is the primary site of MBL synthesis, and points toup-regulation asaresult of theviral infections, and the location outside the liver could indicate a role ofMBL intheimmunedefence. Expand