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Agrobacterium-mediated transformation of citrange: factors affecting transformation and regeneration
TLDR
The effects of cocultivation with Agrobacterium tumefaciens, regeneration and selection conditions on the transformation efficiency of citrange, including the number of regenerated escape shoots, have been investigated. Expand
High efficiency Agrobacterium-mediated transformation and regeneration of citrus
TLDR
The transformation efficiencies obtained are the highest reported so far for citrus, and extended time periods of selection greatly improved recovering of transformed shoots and reduced the occurrence of escapes. Expand
Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants
TLDR
This transformation procedure showed the highest transgenic plant production efficiency reported for Citrus, and was confirmed by Southern analysis. Expand
Genetic transformation of lime (Citrus aurantifolia Swing.): factors affecting transformation and regeneration
TLDR
Optimum regeneration of transgenic shoots was obtained by exposing the explants to darkness for 2 weeks and by using kanamycin at 100 mg/l as selective agent and attempts to use geneticin as selection antibiotic were not successful. Expand
Constitutive expression of Arabidopsis LEAFY or APETALA1 genes in citrus reduces their generation time
TLDR
Transforming juvenile citrus seedlings to constitutively express the Arabidopsis LEAFY or APETALA1 (AP1) genes resulted in two types of transgenic citrus trees that produced fertile flowers and fruits as early as the first year through a mechanism involving an appreciable shortening of their juvenile phase. Expand
Genetic transformation and regeneration of mature tissues of woody fruit plants bypassing the juvenile stage
TLDR
For the first time, a procedure for genetic transformation and regeneration of mature tissues of woody plants that overcomes the long juvenile periods and high heterozygosity that are characteristic of most of these species is reported. Expand
Sequencing of diverse mandarin, pummelo and orange genomes reveals complex history of admixture during citrus domestication
TLDR
This work sequence and compare citrus genomes—a high-quality reference haploid clementine genome and mandarin, pummelo, sweet-orange and sour-orange genomes—and shows that cultivated types derive from two progenitor species. Expand
Early events in Agrobacterium-mediated genetic transformation of citrus explants.
TLDR
It is demonstrated that cells competent for transformation are located in the newly formed callus growing from the cambial ring of citrus explants, and use of proper co-cultivation medium and conditions led to a higher number of stably transformed cells and to an increase in the final number of regenerated transgenic plants. Expand
Complex history of admixture during citrus domestication revealed by genome analysis
TLDR
The genomes of a collection of mandarins, pummelos, and oranges are reported, including a high quality reference sequence from a haploid Clementine mandarin, and it is shown that these cultivated types can be derived from two progenitor species. Expand
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