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Droplet Barcoding for Single-Cell Transcriptomics Applied to Embryonic Stem Cells
It has long been the dream of biologists to map gene expression at the single-cell level. With such data one might track heterogeneous cell sub-populations, and infer regulatory relationships between… Expand
Single-cell barcoding and sequencing using droplet microfluidics
Single-cell RNA sequencing has recently emerged as a powerful tool for mapping cellular heterogeneity in diseased and healthy tissues, yet high-throughput methods are needed for capturing the… Expand
Single-cell analysis and sorting using droplet-based microfluidics
- L. Mazutis, J. Gilbert, W. L. Ung, D. Weitz, A. Griffiths, J. Heyman
- Biology, Medicine
- Nature Protocols
- 1 May 2013
We present a droplet-based microfluidics protocol for high-throughput analysis and sorting of single cells. Compartmentalization of single cells in droplets enables the analysis of proteins released… Expand
Quantitative and sensitive detection of rare mutations using droplet-based microfluidics.
Somatic mutations within tumoral DNA can be used as highly specific biomarkers to distinguish cancer cells from their normal counterparts. These DNA biomarkers are potentially useful for the… Expand
Multi-step microfluidic droplet processing: kinetic analysis of an in vitro translated enzyme.
Microdroplets in water-in-oil emulsions can be used as microreactors with volumes 10(3) to 10(9) times smaller than the smallest working volumes in a microtitre plate well (1-2 microL). However, many… Expand
Droplet-based microfluidic systems for high-throughput single DNA molecule isothermal amplification and analysis.
- L. Mazutis, A. Araghi, +9 authors Michael Ryckelynck
- Chemistry, Medicine
- Analytical chemistry
- 18 May 2009
We have developed a method for high-throughput isothermal amplification of single DNA molecules in a droplet-based microfluidic system. DNA amplification in droplets was analyzed using an… Expand
A fast and efficient microfluidic system for highly selective one-to-one droplet fusion.
Microdroplets in microfluidic systems can be used as independent microreactors to perform a range of chemical and biological reactions. However, in order to add new reagents to pre-formed droplets at… Expand
Transcriptional Basis of Mouse and Human Dendritic Cell Heterogeneity
Summary Dendritic cells (DCs) play a critical role in orchestrating adaptive immune responses due to their unique ability to initiate T cell responses and direct their differentiation into effector… Expand
Biocompatible fluorinated polyglycerols for droplet microfluidics as an alternative to PEG-based copolymer surfactants.
In droplet-based microfluidics, non-ionic, high-molecular weight surfactants are required to stabilize droplet interfaces. One of the most common structures that imparts stability as well as… Expand
DNA Nanoparticles for Improved Protein Synthesis In Vitro
- Robertas Galinis, G. Stonyte, +5 authors L. Mazutis
- Medicine, Chemistry
- Angewandte Chemie
- 28 January 2016
Abstract The amplification and digital quantification of single DNA molecules are important in biomedicine and diagnostics. Beyond quantifying DNA molecules in a sample, the ability to express… Expand