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Alterations in the protein composition of maturing phagosomes.
TLDR
Although the macrophage phagosome's total protein composition changes little during phagolysosome formation, the maturing phagosomes both receives and eliminates proteins, possibly by protein recycling, specific membrane and sequestered proteins. Expand
Acrosomal exocytosis, a special type of regulated secretion
TLDR
The acrosome is a single secretory granule present in the head of mammalian ‐ and other animal groups ‐ sperm and because of its particular characteristics, acrosomal exocytosis is a unique mammalian model for the study of the different steps of the membrane fusion cascade. Expand
Dynamics of SNARE Assembly and Disassembly during Sperm Acrosomal Exocytosis
TLDR
It is shown that, in unactivated sperm, SNAREs are locked in heterotrimeric cis complexes, and the unidirectional and sequential disassembly and assembly of SNARE complexes drive acrosomal exocytosis. Expand
On the killing of mycobacteria by macrophages
TLDR
EM analysis showed clearly that all the mycobacteria remained within phagosomes even at late times of infection, and theoretical modelling of the data identified two different potential sets of explanations that are consistent with the results. Expand
Calcium-induced Acrosomal Exocytosis Requires cAMP Acting through a Protein Kinase A-independent, Epac-mediated Pathway*
TLDR
CAMP-elicited exocytosis was sensitive to anti-α-SNAP, anti-NSF, and anti-Rab3A antibodies, to intra-acrosomal Ca2+ chelators, and to botulinum toxins but was resistant to cAMP-dependent protein kinase blockers. Expand
Rab22a Regulates the Sorting of Transferrin to Recycling Endosomes
TLDR
It is speculated that Rab22a controls the transport of the transferrin receptor from sorting to recycling endosomes and depletion by small interfering RNA disorganized the perinuclear recycling center and strongly inhibited transferrin recycling. Expand
PTP1B Dephosphorylates N-Ethylmaleimide-sensitive Factor and Elicits SNARE Complex Disassembly during Human Sperm Exocytosis*
TLDR
It is demonstrated that phosphorylation of NSF on tyrosine residues prevents its SNARE complex dissociation activity and establish for the first time a role for PTP1B in the modulation of the membrane fusion machinery. Expand
Transport of phagosomal components to an endosomal compartment.
TLDR
Characterization of the fusogenic properties of the transport vesicles demonstrated that they are capable of in vitro fusion with early endosomes, and Morphological analysis of phagosome maturation provides additional evidence for phagocytosed to endosome transport. Expand
Epac Activates the Small G Proteins Rap1 and Rab3A to Achieve Exocytosis*
TLDR
It is proposed that Epac sits at a critical point during the exocytotic cascade after which the pathway splits into two limbs, one that assembles the fusion machinery into place and another that elicits intracellular calcium release. Expand
Membrane trafficking along the phagocytic pathway.
TLDR
In vitro reconstitution and other studies suggest that the trafficking events underlying phagosome maturation require several GTP-binding proteins, including Rab5 and Galphas', NSF-SNAP-SNARE complexes and coatomers. Expand
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