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Siderophore‐mediated cell signalling in Pseudomonas aeruginosa: divergent pathways regulate virulence factor production and siderophore receptor synthesis
TLDR
This work shows that a second divergent branch of this signalling pathway regulates the production of the FpvA protein, the first example of a branched signalling system of this sort and the first examples of an antisigma factor protein (FpvR) that directly regulates the activities of two different ECF sigma factor proteins (PvdS and FpVI). Expand
Synthesis of the siderophore pyoverdine in Pseudomonas aeruginosa involves a periplasmic maturation
TLDR
Using the fluorescent properties of PVDI, the pyoverdine produced by Pseudomonas aeruginosa PAO1, it was able to show that PVD i was not present in the cytoplasm of the bacteria, but large amounts of a fluorescent PVDi precursor PVD Ip were stored in the periplasm. Expand
Identification and characterization of novel pyoverdine synthesis genes in Pseudomonas aeruginosa.
TLDR
It is likely that most if not all of the genes required for pyoverdine synthesis in P. aeruginosa PAO1 have now been identified and this will form the basis for a biochemical description of the pathway of pyoverDine synthesis. Expand
Differential proteolysis of sigma regulators controls cell‐surface signalling in Pseudomonas aeruginosa
TLDR
Findings in the pyoverdine, ferrichrome and desferrioxamine siderophore systems of Pseudomonas aeruginosa indicate that regulated proteolysis is a general mechanism for signal transduction in cell‐surface signalling. Expand
Pseudomonas aeruginosa Uses Multiple Pathways To Acquire Iron during Chronic Infection in Cystic Fibrosis Lungs
TLDR
The data show that multiple P. aeruginosa iron uptake mechanisms are active in chronic CF infection and that RT-qPCR of RNA extracted from sputum provides a powerful tool for investigating bacterial physiology during infection in CF. Expand
An efflux pump is involved in secretion of newly synthesized siderophore by Pseudomonas aeruginosa
TLDR
Pseudomonas aeruginosa secretes the fluorescent siderophore, pyoverdine (PVD), to enable iron acquisition and the role of an efflux pump, PvdRT‐OpmQ, in PVD secretion was investigated. Expand
Characterization of a gene encoding an acetylase required for pyoverdine synthesis in Pseudomonas aeruginosa.
TLDR
Characterization of pvdY(II) provides insights into the molecular basis for production of different pyoverdines by different strains of P. aeruginosa, and indicates that the PvdYII enzyme catalyzes acetylation of hydroxyornithine. Expand
Pseudomonas siderophores in the sputum of patients with cystic fibrosis
TLDR
The data show that pyoverdine is an important iron-scavenging molecule for P. aeruginosa in many cystic fibrosis patients, but other P. Aerug inosa iron-uptake systems must be active in some patients to satisfy the bacterial need for iron. Expand
An efflux pump is required for siderophore recycling by Pseudomonas aeruginosa.
TLDR
Data show that PvdRT-OpmQ is involved in recycling of PVDI from the periplasm to the extracellular medium and recycling requires release of the metal ion from PVDi. Expand
Interactions between an anti-sigma protein and two sigma factors that regulate the pyoverdine signaling pathway in Pseudomonas aeruginosa
TLDR
Data show that FpvR binds to PvdS in both P. aeruginosa and E. coli, inhibiting its activity and suggesting that they increase either the stability of Pvd S or its affinity for core RNA polymerase. Expand
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