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Phylogeny of the Mycoplasma mycoides cluster based on analysis of five conserved protein-coding sequences and possible implications for the taxonomy of the group.
TLDR
A phylogenetic tree of the Mycoplasma mycoides cluster was inferred from a set of concatenated sequences from five housekeeping genes, which allowed us to assign phylogenetic positions to all members of the M. my coides cluster and related species and has proved the need to adjust the existing taxonomy. Expand
Mycoplasma leachii sp. nov. as a new species designation for Mycoplasma sp. bovine group 7 of Leach, and reclassification of Mycoplasma mycoides subsp. mycoides LC as a serovar of Mycoplasma mycoides
TLDR
The Mycoplasma mycoides cluster consists of six pathogenic mycoplasmas causing disease in ruminants, which share many genotypic and phenotypic traits and the present paper proposes an emended taxonomy of this cluster, which will hereafter be composed of five taxa comprising three subclusters. Expand
A specific PCR for the identification of Mycoplasma capricolum subsp. capripneumoniae, the causative agent of contagious caprine pleuropneumonia (CCPP).
TLDR
A specific polymerase chain reaction was developed for the surveillance of contagious caprine pleuropneumonia and it was validated on 14 M. capripneumoniae strains and 27 heterologous strains belonging to the "M. mycoides cluster". Expand
Multi-locus sequence analysis of mycoplasma capricolum subsp. capripneumoniae for the molecular epidemiology of contagious caprine pleuropneumonia
TLDR
The presence of a distinct Asian cluster strongly indicates that CCPP was not recently imported to continental Asia and is more likely that the disease has been endemic in the area for a long time, as supported by historical clinical descriptions. Expand
Detection of contagious caprine pleuropneumonia in East Turkey.
TLDR
The results showed that the agent of CCPP, Mycoplasma capricolum subspecies capripneumoniae (Mccp), could be detected by culture and specific polymerase chain reaction from 37.5% of lung samples taken from goats of ten different herds, bringing the strict host-specificity of this pathogen into question. Expand
Phylogeny and molecular typing of Mycoplasma agalactiae and Mycoplasma bovis by multilocus sequencing.
TLDR
The MLST scheme developed here constitutes a universal tool for unequivocal strain characterisation and global, long-term screening of dissemination of M. agalactiae and M. bovis, whereas addition of more variable, non-housekeeping gene targets allows precise epidemiological investigations. Expand
An international collaborative study to determine the prevalence of contagious caprine pleuropneumonia by monoclonal antibody-based cELISA
TLDR
A new competitive ELISA kit for CCPP, based on a previous blocking ELISA, was formatted and used to evaluate the prevalence of CCPP in some regions of Kenya, Ethiopia, Mauritius, Tajikistan and Pakistan in an international collaborative study and retained the high specificity of the original kit. Expand
Characterization of Free Exopolysaccharides Secreted by Mycoplasma mycoides Subsp. mycoides
TLDR
A method to produce large quantities of EPS by transfer of mycoplasma cells from their complex broth to a chemically defined medium and subsequent purification and in silico analyses of candidate polysaccharide biosynthetic pathways in order to decipher the potential link between glucose phosphotransferase permease activity and attachment/release of galactan. Expand
Genotyping of Mycoplasma mycoides subsp. mycoides SC by multilocus sequence analysis allows molecular epidemiology of contagious bovine pleuropneumonia.
TLDR
Cluster analysis confirmed that European strains from the 1990s did not originate from Africa and showed a genetic link between a European strain isolated in 1967 and those found in southern Africa and Australia, in agreement with historical data showing that CBPP was introduced in these regions during colonisation in the 19th century. Expand
Evolutionary History of Contagious Bovine Pleuropneumonia Using Next Generation Sequencing of Mycoplasma mycoides Subsp. mycoides “Small Colony”
TLDR
A large-scale genomic approach based on next-generation sequencing technologies was applied to construct a robust phylogeny of this extremely monomorphic pathogen, which identified a lineage specific to European strains; African strains were scattered in various branches. Expand
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