MicroRNA targeting specificity in mammals: determinants beyond seed pairing.
Prediction of Plant MicroRNA Targets
Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs
These results suggest that metazoan miRNAs can reduce the levels of many of their target transcripts, not just the amount of protein deriving from these transcripts, and seem to downregulate a far greater number of targets than previously appreciated.
An Abundant Class of Tiny RNAs with Probable Regulatory Roles in Caenorhabditis elegans
Two small temporal RNAs, lin-4 andlet-7, control developmental timing in Caenorhabditis elegans and are found to be members of a large class of 21- to 24-nucleotide noncodingRNAs, called microRNAs (miRNAs), which imply that, as a class, miRNAs have broad regulatory functions in animals.
The microRNAs of Caenorhabditis elegans.
The census of the worm miRNAs and their expression patterns helps define this class of noncoding RNAs, lays the groundwork for functional studies, and provides the tools for more comprehensive analyses of miRNA genes in other species.
A microRNA component of the p53 tumour suppressor network
A family of miRNAs, miR-34a–c, whose expression reflected p53 status is described, whose encoded genes are direct transcriptional targets of p53, whose induction by DNA damage and oncogenic stress depends on p53 both in vitro and in vivo.
Computational inference of homologous gene structures in the human genome.
A new gene identification algorithm, GenomeScan, which combines exon-intron and splice signal models with similarity to known protein sequences in an integrated model, which shows an accurate and efficient automated approach for identifying genes in higher eukaryotic genomes and provide a first-level annotation of the draft human genome.
Transcripts Targeted by the MicroRNA-16 Family Cooperatively Regulate Cell Cycle Progression
It is demonstrated that a family of miRNAs sharing sequence identity with miRNA-16 (miR-16) negatively regulates cellular growth and cell cycle progression and coordinately regulates targets that may act in concert to controlcell cycle progression.
Vertebrate MicroRNA Genes
A computational procedure (MiRscan) is developed to identify miRNA genes and applies it here to miRNAs known to control the expression of other genes at the posttranscriptional level.
The Widespread Impact of Mammalian MicroRNAs on mRNA Repression and Evolution
It is found that these conserved targets are often highly expressed at developmental stages before miRNA expression and that their levels tend to fall as the miRNA that targets them begins to accumulate.