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Role of free L-carnitine and acetyl-L-carnitine in post-gonadal maturation of mammalian spermatozoa.
The potential to initiate sperm motility, which takes place in the epididymis, is probably independent of the carnitine system, while the energy properties of acetyl-L-carnitine can only be relevant in situations of "energy crisis'. Expand
Evaluation of chromatin condensation in human spermatozoa: a flow cytometric assay using acridine orange staining.
The use of swim-up and Percoll gradient centrifugation methods was shown to improve the percentage of spermatozoa with normal chromatin structure in some samples with poor initial quality. Expand
Concurrent use of flow cytometry and fluorescence in-situ hybridization techniques for detecting faulty meiosis in a human sperm sample.
The use of flow cytometry and FISH in cases where genetic and developmental chromatin abnormalities are suspected is a valuable adjunct to other available techniques, and can guide the clinicians to decide which samples are unsuitable for intracytoplasmic injection. Expand
Flow cytometry of human semen: a preliminary study of a non-invasive method for the detection of spermatogenetic defects.
- N. Levek-Motola, Y. Soffer, L. Shochat, A. Raziel, L. Lewin, R. Golan
- Biology, Medicine
- Human reproduction
- 1 December 2005
The flow cytometric method differentiated between cases of 'Sertoli cell-only' syndrome (complete absence of tetraploid and haploid cells) and cases where spermatogenesis was blocked in meiosis or in spermiogenesis. Expand
Use of confocal microscopy for the study of spermatogenesis.
- M. Vigodner, L. Lewin, T. Glaser, L. Shochat, L. Mittelman, R. Golan
- Biology, Medicine
- Methods in cell science : an official journal of…
LSCM analysis of c-kit and SC3 protein expression at different stages of hamster spermatogenesis is demonstrated, and detailed examination of s permatogonia, sperMatocytes and sper Matids during golden hamsters sper matogenesis is presented. Expand
Studies on the metabolic role of myo-inositol. Distribution of radioactive myo-inositol in the male rat.
The radioactive material present in liver, spleen, brain, heart, diaphragm, seminal vesicle, coagulating gland, prostate, epididymis, vas deferens and testis was shown to consist exclusively of myo-inositol and its derivatives, as shown by paper chromatography of hydrolysates and trichloroacetic acid extracts of these tissues. Expand
Changes in chromatin condensation of human spermatozoa during epididymal transit as determined by flow cytometry.
It may be concluded that eggs fertilized by ejaculated spermatozoa receive a more highly condensed form of chromatin than that received by eggs inseminated with proximal epididymal or testicular spermutozoa. Expand
Carnitine and acylcarnitines in semen from azoospermic patients.
Treatment with human chorionic gonadotropin increased serum testosterone levels in azoospermic patients, but it did not increase the free carnitine concentration in semen, although it increased the proportion of carnitines found in acylcarnitines. Expand
Epididymis and seminal vesicle as sources of carnitine in human seminal fluid: the clinical significance of the carnitine concentration in human seminal fluid.
Carnitine determinations in human seminal fluid were shown to be useful in assessing epididymal and seminal vesicle function and in locating blockages in the male reproductive tract and the possible role of carnitine in sperm maturation was discussed. Expand
In-vitro human spermatozoa nuclear decondensation assessed by flow cytometry.
- D. Samocha-Bone, L. Lewin, +4 authors R. Golan
- Biology, Medicine
- Molecular human reproduction
- 1 February 1998
In cases where spermatozoa fail to produce a fertilized egg the cause may lie with defective chromatin quality, including failure of the sperm chromatin to decondense, the method described here is a simple procedure for detecting sperm samples containing such defective cells. Expand