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PCR-Based Diagnosis of Acute and Chronic Cutaneous Leishmaniasis Caused by Leishmania (Viannia)
ABSTRACT We evaluated PCR methods for diagnosis of acute and chronic cutaneous leishmaniasis (CL) in an area of Colombia where Leishmania (Viannia) is endemic. The PCR method specifically amplifiedExpand
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Evaluation of polymerase chain reaction, adenosine deaminase, and interferon-gamma in pleural fluid for the differential diagnosis of pleural tuberculosis.
STUDY OBJECTIVES Pleural tuberculosis (TB) is a diagnostic challenge because of its nonspecific clinical presentation and paucibacillary nature. The inefficiency of conventional laboratory methodsExpand
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A comparative study of diagnosis by the polymerase chain reaction and by current clinical methods using biopsies from Colombian patients with suspected leishmaniasis.
The results of a preliminary trial are reported in which the diagnostic value of a Polymerase Chain Reaction (PCR) specific for Leishmania of the subgenus Viannia was compared with that of currentlyExpand
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Recurrent lesions in human Leishmania braziliensis infection—reactivation or reinfection?
Strains of Leishmania braziliensis subspecies isolated from initial and recurrent lesions in 24 patients from the Pacific coast of Colombia were examined for distinguishing polymorphisms by enzymeExpand
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Photographic and Luminometric Detection of Luciferase Reporter Phages for Drug Susceptibility Testing of Clinical Mycobacterium tuberculosis Isolates
ABSTRACT Luciferase reporter phages (LRPs) have proven to be efficient tools for drug susceptibility testing of Mycobacterium tuberculosis. Luminometric detection of LRP activity offers higherExpand
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Evaluation of Etest for Susceptibility Testing of Multidrug-Resistant Isolates of Mycobacterium tuberculosis
ABSTRACT To prescribe effective treatment schemes for patients with tuberculosis, more-efficient susceptibility testing techniques forMycobacterium tuberculosis are needed, especially in regions withExpand
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Stained smears as a source of DNA.
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Resumen del taller sobre el uso de la reacción en cadena de la polimerasa (PCR) para distinguir entre Trypanosoma cruzi y tripanosoma rangeli
Este taller se realizo con el proposito de transferir la tecnologia de la reaccion en cadena de la polimerasa (PCR) para la deteccion de T. cruzi y T. rangeli a laboratorios en Colombia involucradosExpand
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Minicircle variable region probes for characterization of Leishmania (Viannia) species.
The minicircle molecules present in the kinetoplast DNA (kDNA) network constitute a particularly useful molecular tool because they are a multicopy target and present a variable region that differsExpand
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Frequency of the Asn-108 and Thr-108 point mutations in the dihydrofolate reductase gene in Plasmodium falciparum from southwest Colombia.
Several point mutations in the dihydrofolate reductase (DHFR) gene of Plasmodium falciparum have been correlated with in vitro anti-folate drug resistance of laboratory and field isolates.Expand
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