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Endoplasmic Reticulum Export Sites and Golgi Bodies Behave as Single Mobile Secretory Units in Plant Cells
TLDR
It is proposed that punctate accumulation of Sar1p represents ER export sites (ERES), and this work could demonstrate using the drug brefeldin A that formation of ERES is strictly dependent on a functional retrograde transport route from the Golgi apparatus. Expand
Receptor Salvage from the Prevacuolar Compartment Is Essential for Efficient Vacuolar Protein Targeting
TLDR
It is proposed that recycling of the vacuolar sorting receptor from the prevacuolar compartment to the Golgi apparatus is an essential process that is saturable and wortmannin sensitive. Expand
Secretory Bulk Flow of Soluble Proteins Is Efficient and COPII Dependent
TLDR
An in vivo assay in which COPII vesicle transport is disrupted at two distinct steps in the pathway is developed, demonstrating that export of the ER resident protein calreticulin or the bulk flow marker phosphinothricin acetyl transferase is COPII dependent and occurs at a much higher rate than estimated previously. Expand
Targeting of the Plant Vacuolar Sorting Receptor BP80 Is Dependent on Multiple Sorting Signals in the Cytosolic Tail[W]
TLDR
It is shown that both the transmembrane domain and the cytosolic tail of BP80 contain information necessary for efficient progress to the prevacuolar compartment (PVC) and the tail must be exposed on the correct membrane surface to compete with sorting machinery. Expand
ER quality control can lead to retrograde transport from the ER lumen to the cytosol and the nucleoplasm in plants.
TLDR
The data are consistent with the hypothesis that the fusion protein is disposed off from the ER via a retrograde translocation back to the cytosol, and accumulation in the nucleoplasm was shown to be microtubule dependent unlike the well-documented diffusion of cytosolsolically expressed GFP into theucleoplasm. Expand
Overexpression of the Arabidopsis Syntaxin PEP12/SYP21 Inhibits Transport from the Prevacuolar Compartment to the Lytic Vacuole in Vivo[W]
TLDR
It is concluded that PEP12/SYP21 overexpression specifically inhibits export from the PVC without affecting the Golgi complex or compromising the secretory branch of the endomembrane system. Expand
Golgi-Dependent Transport of Vacuolar Sorting Receptors Is Regulated by COPII, AP1, and AP4 Protein Complexes in Tobacco[W][OPEN]
TLDR
It is proposed that cycling via the plasma membrane is unlikely to play a role in biosynthetic vacuolar sorting under normal physiological conditions and that the conserved Ile-Met motif is mainly used to recover mistargeted receptors. Expand
cDNA cloning and functional expression of KM+, the mannose-binding lectin from Artocarpus integrifolia seeds.
TLDR
Analysis of KM+ nucleotide and deduced amino acid sequences provided strong evidence that it neither enters the secretory pathway nor undergoes post-translational modifications, which is in sharp contrast with jacalin, the more abundant lectin from A. integrifolia seeds. Expand
A tobacco cDNA reveals two different transcription patterns in vegetative and reproductive organs.
TLDR
The results suggest that the shorter transcript is related to stress while the larger one is a flower predominant and nonstress-inducible messenger. Expand