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A method for designing primer sets for speciation studies in filamentous ascomycetes
A simple method is described for designing primer sets that can amplify specific protein-encoding sequences in a wide variety of filamentous ascomycetes. Using this technique, we successfully desig...
Genomic Analysis of the Necrotrophic Fungal Pathogens Sclerotinia sclerotiorum and Botrytis cinerea
Comparative genome analysis revealed the basis of differing sexual mating compatibility systems between S. sclerotiorum and B. cinerea, and shed light on the evolutionary and mechanistic bases of the genetically complex traits of necrotrophic pathogenicity and sexual mating. Expand
A multilocus gene genealogy concordant with host preference indicates segregation of a new species, Magnaporthe oryzae, from M. grisea.
Gene trees were inferred for Magnaporthe species using portions of three genes: actin, beta-tubulin, and calmodulin and distinguished two distinct clades within M. grisea. Expand
Mycelial incompatibility and molecular markers identify genetic variability in field populations of Sclerotinia sclerotiorum
- L. Kohn
Sclerotial strains of Sclerotinia were obtained from transects in two fields of canola in Ontario and six mycelial compatibility groups (MCGs) were recognized, the largest group including 19 strains from the first field. Expand
Local and trans-Canadian clonal distribution of Sclerotinia sclerotiorum on canola
Clonal variability within and among field populations of Sclerotinia sclerotiorum isolated from canola petals in western Canada was determined by analysis of two independent criteria, mycelial… Expand
Origins of Host-Specific Populations of the Blast Pathogen Magnaporthe oryzae in Crop Domestication With Subsequent Expansion of Pandemic Clones on Rice and Weeds of Rice
The rice-infecting lineage is characterized by high copy number of the transposable element MGR586 (Pot3) and by a loss of AVR-Co39, and host-haplotype association is evidenced by low pathogenicity on hosts associated with other haplotypes. Expand
Clonality in Sclerotinia sclerotiorum on Infected Cabbage in Eastern North Carolina.
The North Carolina sample had a clonal component, but deviated from one-to-one association of MCG with DNA fingerprint to an extent consistent with more recombination or transposition than the other two populations sampled. Expand
The clonal dynamic in wild and agricultural plant–pathogen populations
- L. Kohn
- 31 December 1995
The stability or change in clone frequencies during the disease cycle and from year to year is what I term the clonal dynamic. Among pathogenic fungi, the prevalence of efficient asexual reproduction… Expand
Clonal dispersal and spatial mixing in populations of the plant pathogenic fungus, Sclerotinia sclerotiorum
Nine of the 30 most frequently sampled clones from this study were previously recovered in a macrogeographical sample from western Canada in 1990, suggesting spatial mixing of ascospore inoculum from resident or immigrant sources. Expand
Incipient speciation by divergent adaptation and antagonistic epistasis in yeast
The results show that adaptation to divergent environments promotes the evolution of reproductive isolation through antagonistic epistasis, providing evidence of a plausible common avenue to speciation and adaptive radiation in nature. Expand