• Publications
  • Influence
Production of pharmaceutical proteins by transgenic animals
  • L. Houdebine
  • Biology, Medicine
  • Comparative Immunology, Microbiology and…
  • 19 February 2008
Abstract Proteins started being used as pharmaceuticals in the 1920s with insulin extracted from pig pancreas. In the early 1980s, human insulin was prepared in recombinant bacteria and it isExpand
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Animal Transgenesis and Cloning
INTRODUCTION. CHAPTER 1 FROM THE GENE TO THE TRANSGENIC ANIMAL. Genome composition. Gene structure. The number of genes in genomes. The major techniques of genetic engineering. The systematicExpand
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Recombinant human extracellular superoxide dismutase produced in milk of transgenic rabbits
Expression of human extracellular superoxide dismutase (EC-SOD), a glycosylated, tetrameric metalloprotein, was targeted to the lactating mammary gland of transgenic rabbits. Efficient expression ofExpand
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High level production of human growth hormone in the milk of transgenic mice: the upstream region of the rabbit whey acidic protein (WAP) gene targets transgene expression to the mammary gland
The 5′ flanking region (6.3 kb) of the rabbit WAP (rWAP) gene possesses important regulatory elements. This region was linked to the human growth hormone (hGH) structural gene in order to targetExpand
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The Potential Benefits of Insulators on Heterologous Constructs in Transgenic Animals
Transgenes can display position effects, shown as variegated, ectopic, uncontrolled and even silenced expression. These undesired side effects result in irreproducible and non-systematic expressionExpand
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The methods to generate transgenic animals and to control transgene expression.
  • L. Houdebine
  • Biology, Medicine
  • Journal of biotechnology
  • 25 September 2002
Transgenic animals have been used for years to study gene function and to create models for the study of human diseases. This approach has become still more justified after the complete sequencing ofExpand
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Transgenic animal models in biomedical research.
  • L. Houdebine
  • Biology, Medicine
  • Methods in molecular biology
  • 2007
Transgenic animals have become a key tool in functional genomics to generate models for human diseases and validate new drugs. Transgenesis includes the addition of foreign genetic information toExpand
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Comparison of insulin-like growth factor 1 and insulin effects on prolactin-induced lactogenesis in the rabbit mammary gland in vitro
In organ culture of pregnant rabbit mammary gland, a low casein synthesis occurs with prolactin (PRL) alone. Insulin markedly potentiates the effect of PRL. Only pharmacological concentrations ofExpand
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Worsening of Diet-Induced Atherosclerosis in a New Model of Transgenic Rabbit Expressing the Human Plasma Phospholipid Transfer Protein
Objective—Plasma phospholipid transfer protein (PLTP) is involved in intravascular lipoprotein metabolism. PLTP is known to act through 2 main mechanisms: by remodeling high-density lipoproteinsExpand
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Effect of the Rabbit αs1-Casein Gene Distal Enhancer on the Expression of a Reporter Gene in Vitro and in Vivo
Abstract Several gene constructs containing the firefly luciferase gene and the herpes simplex virus thymidine kinase gene promoter (TK) were used to evaluate the transcriptional activity of theExpand
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