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Direct multiplexed measurement of gene expression with color-coded probe pairs
TLDR
Comparisons of transcript levels for 21 genes across seven samples measured by the nCounter system and SYBR Green real-time PCR demonstrated similar patterns of gene expression at all transcript levels, demonstrating that the n counter system is more sensitive than microarrays and similar in sensitivity to real- time PCR.
Ataxia in prion protein (PrP)-deficient mice is associated with upregulation of the novel PrP-like protein doppel.
TLDR
Dpl is the first PrP-like protein to be described in mammals, and since Dpl seems to cause neurodegeneration similar to PrP, the linked expression of the Prnp and Prnd genes may play a previously unrecognized role in the pathogenesis of prion diseases or other illnesses.
A Genomic Regulatory Network for Development
TLDR
A gene regulatory network that controls the specification of endoderm and mesoderm in the sea urchin embryo is summarized here and reveals specific and general aspects of development, such as how given cells generate their ordained fates in the embryo and why the process moves inexorably forward in developmental time.
A common language for physical mapping of the human genome.
TLDR
The polymerase chain reaction (PCR), a method that has only come into widespread use during the past 2 years, seems to offer a path toward a physical map that largely circumvents two problems that were prominent in the NRC Committee's discussions.
Fluorescence detection in automated DNA sequence analysis
TLDR
A method for the partial automation of DNA sequence analysis by means of a fluorophore covalently attached to the oligonucleotide primer used in enzymaticDNA sequence analysis.
A ligase-mediated gene detection technique.
TLDR
An assay for the presence of given DNA sequences has been developed, based on the ability of two oligonucleotides to anneal immediately adjacent to each other on a complementary target DNA molecule, which permits the rapid and standardized identification of single-copy gene sequences in genomic DNA.
Simian sarcoma virus onc gene, v-sis, is derived from the gene (or genes) encoding a platelet-derived growth factor.
TLDR
The demonstrating of extensive sequence similarity between the transforming protein derived from the simian sarcoma virus onc gene, v-sis, and a human platelet-derived growth factor shows that this protein could be a factor active transiently during normal cell growth.
Structural studies of the scrapie prion protein using mass spectrometry and amino acid sequencing.
TLDR
This work contends that PrPSc molecules do not differ from PrPC at the level of an amino acid substitution or a posttranslational chemical modification; however, it cannot eliminate the possibility that a small fraction ofPrPSc is modified by an as yet unidentified posttranslated process or that PrPC carries a modification that is removed in the formation of PrP Sc.
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