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The Effect of Geographical Scale of Sampling on DNA Barcoding
A CO1 data set of aquatic predaceous diving beetles of the tribe Agabini is presented and it is shown that even if samples are collected to maximize the geographical coverage, up to 70 individuals are required to sample 95% of intraspecific variation, showing that the geographical scale of sampling has a critical impact on the global application of DNA barcoding.
A comprehensive DNA barcode database for Central European beetles with a focus on Germany: adding more than 3500 identified species to BOLD
- L. Hendrich, J. Morinière, M. Balke
- Biology, Environmental ScienceMolecular Ecology Resources
- 1 July 2015
This study provides the globally largest DNA barcode reference library for Coleoptera for 15 948 individuals belonging to 3514 well‐identified species (53% of the German fauna) with representatives from 97 of 103 families with a focus on Germany.
Building-Up of a DNA Barcode Library for True Bugs (Insecta: Hemiptera: Heteroptera) of Germany Reveals Taxonomic Uncertainties and Surprises
- M. Raupach, L. Hendrich, S. Küchler, Fabian Deister, J. Morinière, M. Gossner
- BiologyPLoS ONE
- 9 September 2014
This study test the efficiency of DNA barcoding for true bugs (Hemiptera: Heteroptera), an ecological and economical highly important as well as morphologically diverse insect taxon, and highlights the urgent necessity of taxonomic revisions for various taxa of the Heterptera.
Mitochondrial Cox1 Sequence Data Reliably Uncover Patterns of Insect Diversity But Suffer from High Lineage-Idiosyncratic Error Rates
Cox1 sequence data are a powerful tool for large-scale species richness estimation, with a great potential for use in ecology and β-diversity studies and for setting conservation priorities, however, error rates can be high in individual lineages.
A DNA barcode library for Germany′s mayflies, stoneflies and caddisflies (Ephemeroptera, Plecoptera and Trichoptera)
- J. Morinière, L. Hendrich, G. Haszprunar
- Biology, Environmental ScienceMolecular ecology resources
- 1 November 2017
Both maximum pairwise and nearest neighbour distances were substantially higher for Ephemeroptera compared to Plecoptera and Trichoptera, possibly indicating older speciation events, stronger positive selection or faster rate of molecular evolution.
Species Identification in Malaise Trap Samples by DNA Barcoding Based on NGS Technologies and a Scoring Matrix
A meta-barcoding pipeline is developed to streamline the barcoding process and indicate that a fast, efficient and reliable analysis of next generation data from malaise trap samples can be achieved using this pipeline.
Towards a DNA Barcode Reference Database for Spiders and Harvestmen of Germany
The overwhelming majority of species could be readily identified through DNA barcoding: median distances between closest species lay around 9% in spiders and 13% in harvestmen, while in 95% of the cases, intraspecific distances were below 2.5%.
A DNA barcode library for 5,200 German flies and midges (Insecta: Diptera) and its implications for metabarcoding‐based biomonitoring
This study provides an intermediate taxonomic system for half of the German Dipteran fauna, which will provide a useful foundation for subsequent detailed, integrative taxonomic studies, and demonstrates that species delineation using BINs and operational taxonomic units (OTUs) constitutes an effective method for biodiversity studies using DNA metabarcoding.
The Trichoptera barcode initiative: a strategy for generating a species-level Tree of Life
- Xin Zhou, P. Frandsen, K. Kjer
- BiologyPhilosophical Transactions of the Royal Society B…
- 5 September 2016
A strategy of implementing constrained tree searches is suggested, which allow larger datasets to dictate the backbone phylogeny, while the barcode data fill out the tips of the tree.
Ecological Niche Modelling and nDNA Sequencing Support a New, Morphologically Cryptic Beetle Species Unveiled by DNA Barcoding
The use of nDNA and bioclimatic modelling in a new species of aquatic beetle revealed by mtDNA sequence data and extended use of mitochondrial sequences shows great potential for delineating morphologically cryptic species.