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The Effect of Geographical Scale of Sampling on DNA Barcoding
TLDR
A CO1 data set of aquatic predaceous diving beetles of the tribe Agabini is presented and it is shown that even if samples are collected to maximize the geographical coverage, up to 70 individuals are required to sample 95% of intraspecific variation, showing that the geographical scale of sampling has a critical impact on the global application of DNA barcoding. Expand
A comprehensive DNA barcode database for Central European beetles with a focus on Germany: adding more than 3500 identified species to BOLD
TLDR
This study provides the globally largest DNA barcode reference library for Coleoptera for 15 948 individuals belonging to 3514 well‐identified species (53% of the German fauna) with representatives from 97 of 103 families with a focus on Germany. Expand
Mitochondrial Cox1 Sequence Data Reliably Uncover Patterns of Insect Diversity But Suffer from High Lineage-Idiosyncratic Error Rates
TLDR
Cox1 sequence data are a powerful tool for large-scale species richness estimation, with a great potential for use in ecology and β-diversity studies and for setting conservation priorities, however, error rates can be high in individual lineages. Expand
Species Identification in Malaise Trap Samples by DNA Barcoding Based on NGS Technologies and a Scoring Matrix
TLDR
A meta-barcoding pipeline is developed to streamline the barcoding process and indicate that a fast, efficient and reliable analysis of next generation data from malaise trap samples can be achieved using this pipeline. Expand
Building-Up of a DNA Barcode Library for True Bugs (Insecta: Hemiptera: Heteroptera) of Germany Reveals Taxonomic Uncertainties and Surprises
TLDR
This study test the efficiency of DNA barcoding for true bugs (Hemiptera: Heteroptera), an ecological and economical highly important as well as morphologically diverse insect taxon, and highlights the urgent necessity of taxonomic revisions for various taxa of the Heterptera. Expand
Ecological Niche Modelling and nDNA Sequencing Support a New, Morphologically Cryptic Beetle Species Unveiled by DNA Barcoding
TLDR
The use of nDNA and bioclimatic modelling in a new species of aquatic beetle revealed by mtDNA sequence data and extended use of mitochondrial sequences shows great potential for delineating morphologically cryptic species. Expand
Evolutionary history of a secondary terrestrial Australian diving beetle (Coleoptera, Dytiscidae) reveals a lineage of high morphological and ecological plasticity
TLDR
The results highlight one of the most remarkable radiations of aquatic beetles with independent ecological pathways likely linked to palaeoclimatic disruptions in the Neogene, and establish a synonymy for both species of Terradessus with Paroster. Expand
New Guinea highland origin of a widespread arthropod supertramp
TLDR
It is shown that an ecologically diverse, common and widespread arthropod supertramp species originated in the highlands of New Guinea, ca 6.0–2.7 Myr ago, which contradicts the widely held view of local endemism as an evolutionary dead end. Expand
A DNA barcode library for Germany′s mayflies, stoneflies and caddisflies (Ephemeroptera, Plecoptera and Trichoptera)
TLDR
Both maximum pairwise and nearest neighbour distances were substantially higher for Ephemeroptera compared to Plecoptera and Trichoptera, possibly indicating older speciation events, stronger positive selection or faster rate of molecular evolution. Expand
From water striders to water bugs: the molecular diversity of aquatic Heteroptera (Gerromorpha, Nepomorpha) of Germany based on DNA barcodes
TLDR
This study demonstrates the usefulness of DNA barcodes for the identification of the aquatic Heteroptera of Germany and adjacent regions and represents an essential baseline for a reference library for bioassessment studies of freshwater habitats using modern high-throughput technologies in the near future. Expand
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