• Publications
  • Influence
Serine protease mechanism and specificity.
  • L. Hedstrom
  • Chemistry, Medicine
  • Chemical reviews
  • 23 November 2002
Almost one-third of all proteases can be classified as serine proteases, named for the nucleophilic Ser residue at the active site. This mechanistic class was originally distinguished by the presenceExpand
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A review of the global burden, novel diagnostics, therapeutics, and vaccine targets for cryptosporidium.
Cryptosporidium spp are well recognised as causes of diarrhoeal disease during waterborne epidemics and in immunocompromised hosts. Studies have also drawn attention to an underestimated globalExpand
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  • PDF
IMP dehydrogenase: structure, mechanism, and inhibition.
  • L. Hedstrom
  • Chemistry, Medicine
  • Chemical reviews
  • 29 May 2009
George Weber was among the first to recognize that extensive metabolic changes must underlie the unbridled proliferation of cancer cells 1. His molecular correlation hypothesis postulated that aExpand
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Azole Drugs Are Imported By Facilitated Diffusion in Candida albicans and Other Pathogenic Fungi
Despite the wealth of knowledge regarding the mechanisms of action and the mechanisms of resistance to azole antifungals, very little is known about how the azoles are imported into pathogenic fungalExpand
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Structural origins of substrate discrimination in trypsin and chymotrypsin.
Converting the specificity of trypsin to that of chymotrypsin has been shown to require the exchange of amino acids in multiple portions of the protein, including two surface loops which do notExpand
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Gene transfer in the evolution of parasite nucleotide biosynthesis.
Nucleotide metabolic pathways provide numerous successful targets for antiparasitic chemotherapy, but the human pathogen Cryptosporidium parvum thus far has proved extraordinarily refractory toExpand
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Inosine 5'-monophosphate dehydrogenase binds nucleic acids in vitro and in vivo.
Inosine 5'-monophosphate dehydrogenase (IMPDH) is the rate-limiting enzyme in the de novo biosynthesis of guanine nucleotides. In addition to the catalytic domain, IMPDH contains a subdomain ofExpand
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The roles of conserved carboxylate residues in IMP dehydrogenase and identification of a transition state analog.
IMP dehydrogenase (IMPDH) catalyzes the oxidation of IMP to XMP with the concomitant reduction of NAD+; the enzyme is activated by K+. This reaction is the rate-limiting step in de novo guanineExpand
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Converting trypsin to chymotrypsin: residue 172 is a substrate specificity determinant.
Trypsin and chymotrypsin have very similar tertiary structures, yet very different substrate specificities. Recent site-directed mutagenesis studies have shown that mutation of the residues of theExpand
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A twisted base? The role of arginine in enzyme-catalyzed proton abstractions.
Arginine residues are generally considered poor candidates for the role of general bases because they are predominantly protonated at physiological pH. Nonetheless, Arg residues have recently emergedExpand
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