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Engineering Herbicide Metabolism in Tobacco and Arabidopsis with CYP76B1, a Cytochrome P450 Enzyme from Jerusalem Artichoke1
TLDR
The data indicate that CYP76B1 can function as a selectable marker for plant transformation, allowing efficient selection in vitro and in soil-grown plants, and may also be a potential tool for phytoremediation of contaminated sites.
Cloning of Arabidopsis thaliana glutathione synthetase (GSH2) by functional complementation of a yeast gsh2 mutant.
TLDR
The yeast gsh2 mutant becomes resistant to methylglyoxal and cadmium after transformation with the plasmid bearing the Arabidopsis GSH2 cDNA, correlated to the restoration of GSH content from below detectability in mutants to about 50% of the wild-type levels in transformed cells.
Regulatory role of microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase in a tobacco mutant that overproduces sterols.
In a tobacco mutant callus, containing up to tenfold more sterols than the wild-type genotype, HMG-CoA reductase activity is increased by a factor of approximately three, as is the case in mutant
Regulation of Sterol Content in Membranes by Subcellular Compartmentation of Steryl-Esters Accumulating in a Sterol-Overproducing Tobacco Mutant
TLDR
The results show that the overproduced sterols of mutant tissues accumulate as steryl-esters in hyaloplasmic bodies, and suggest that the expression of the LAB 1–4 phenotype is dependent on the differentiation state of cells.
Callus induction and establishment of cell suspension cultures of the halophyte Armeria maritima (Mill.) Willd.
Abstract The objective of this work was to establish a cell suspension culture from Armeria maritima (Mill.) Willd., an halophyte so far only valuable for horticultural purposes, as a pre-requisite
Genetic study and further biochemical characterization of a tobacco mutant that overproduces sterols
TLDR
It is demonstrated that a precise regulation of the membrane sterol composition occurs in this tobacco mutant, via a selective esterification process, which indicates that sterols representing the usual end-products of the biosynthetic pathway are preferably integrated into the membranes as free sterols, whereas most of the intermediates pool is esterified and stored in cytoplasmic lipid droplets.
Sterol overproduction is the biochemical basis of resistance to a triazole in calli from a tobacco mutant
TLDR
It is shown that sterol overproduction confers resistance to other phytotoxic sterolbiosynthesis inhibitors, and that the resistance and the biochemical phenotypes co-segregate during meiotic recombination and therefore result most probably from the same mutation.
Activity and concentration of polyphenolic antioxidants and agronomic characteristics of selected cultivars and advanced apple lines from Quebec and British Columbia, Canada
TLDR
The significant variation observed in the agronomic and antioxidant parameters confirms the potential of some of the unnamed lines for processing, use in a breeding program, or both.