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Mechanism of activation of latent recombinant transforming growth factor beta 1 by plasmin
Chemical cross-linking of CHO-conditioned medium and immunoblot analyses indicates that latent recombinant TGF beta 1 contains both the cleaved amino-terminal glycopeptide and mature TGFbeta 1 polypeptide in a noncovalent association and that this association confers latency. Expand
Processing of Transforming Growth Factor 1 Precursor by Human Furin Convertase (*)
Evidence is provided that in experimental systems the TGFβ1 precursor is efficiently and correctly processed by human furin thus permitting release of the biologically active peptide. Expand
Demonstration That Mutation of the Type II Transforming Growth Factor β Receptor Inactivates Its Tumor Suppressor Activity in Replication Error-positive Colon Carcinoma Cells (*)
Results indicate that reconstitution of TGF-β autocrine activity by reexpression of RII can reverse malignancy in RER colon cancers, thus verifying that the malignancies of hereditary nonpolyposis colorectal cancer can be directly associated with the loss of R II expression. Expand
Thrombospondin binds and activates the small and large forms of latent transforming growth factor-beta in a chemically defined system.
The ability of TSP to convert latent T GF-beta to biologically active TGF-beta suggests that TSP is a major regulatory factor in the control of TGF -beta activity. Expand
Molecular events in the processing of recombinant type 1 pre-pro-transforming growth factor beta to the mature polypeptide.
Highly purified recombinant TGF-beta 1 had the same specific biological activity as natural TGF -beta 1 and inhibited mink lung cell proliferation at 50 to 60 pM concentrations. Expand
Identification of mannose 6-phosphate in two asparagine-linked sugar chains of recombinant transforming growth factor-beta 1 precursor.
Recombinant transforming growth factor-beta 1 (TGF- beta 1) precursor produced and secreted by a clone of Chinese hamster ovary cells was found to be glycosylated and phosphorylated and binding studies with the purified receptor for mannose 6-phosphate indicated that the TGF-Beta 1 precursor could bind to this receptor and the binding was specifically inhibited with mannosed 6- phosphate. Expand
Differential stereospecificities and affinities of folate receptor isoforms for folate compounds and antifolates.
Striking differences in the ligand binding sites of MFR-1 and M FR-2 are demonstrated which could potentially be exploited in the design of M FR isoform specific antifolates. Expand
Mechanism of Activation of Latent Recombinant Transforming Growth Factor by Plasmin
Medium conditioned by Chinese hamster ovary (CHO) cells transfected with the simian pre-proTGF/31 cDNA contains high levels of latent TGF/31. The amino-terminal region of the TGF/~I precursor isExpand
The pro domain of pre-pro-transforming growth factor beta 1 when independently expressed is a functional binding protein for the mature growth factor.
Bioassays reveal that the pro domain of pre-pro-TGF-beta 1 forms an inactive complex with exogenously added mature TGF- beta 1, indicating an in vitro structure similar to natural, latent TGF -beta 1 complexes. Expand
Type 1 transforming growth factor beta: amplified expression and secretion of mature and precursor polypeptides in Chinese hamster ovary cells.
The availability of biologically active recombinant type 1 TGF-beta and precursor forms should provide a means to examine the structure, function, and potential in vivo therapeutic use of this growth factor. Expand