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The EVH2 Domain of the Vasodilator-stimulated Phosphoprotein Mediates Tetramerization, F-actin Binding, and Actin Bundle Formation*
Analysis of the functional contribution of highly conserved blocks within this region indicated that residues 259–276 of human VASP are essential for the interaction with F-actin, whereas residues 343–380 are required for tetramerization, probably via coiled-coil formation.
Architecture of the RNA polymerase II–TFIIF complex revealed by cross-linking and mass spectrometry
It is shown that protein cross‐linking coupled to mass spectrometry (MS) has now sufficiently advanced as a tool to extend the Pol II structure to a 15‐subunit, 670 kDa complex of Pol II with the initiation factor TFIIF at peptide resolution.
The Protein Composition of Mitotic Chromosomes Determined Using Multiclassifier Combinatorial Proteomics
An unbiased analysis of the whole chromosome proteome from genetic knockouts of kinetochore protein Ska3/Rama1 revealed that the APC/C and RanBP2/RanGAP1 complexes depend on the Ska complex for stable association with chromosomes.
Extracellular signal‐regulated kinases phosphorylate 5lipoxygenase and stimulate 5‐lipoxygenase product formation in leukocytes
It is demonstrated that extracellular signal‐regulated kinases (ERKs) can phosphorylate 5‐LO in vitro and the data suggest that ERKs and p38 MAPK‐regulated MAPKAPKs can act in conjunction to stimulate 5‐ LO by phosphorylation.
Enzymatic production and complete nuclear magnetic resonance assignment of the sugar lactulose.
Lactulose was unambiguously proved to be the major transglycosylation disaccharide and its molecular structure was investigated in detail and confirmed after purification of the reaction solution by LC-MS and 1D and 2D NMR.
Biodiversity of refrigerated raw milk microbiota and their enzymatic spoilage potential.
In this study, 20 different raw cow's milk samples from single farms and dairy bulk tanks were analysed close to delivery to the dairies or close to processing in the dairy for their cultivable microbiota as well as the lipolytic and proteolytic potential of the isolated microorganisms.
Induction of the d-Amino Acid Oxidase from Trigonopsis variabilis
Induction of the d-amino acid oxidase (EC. from the yeast Trigonopsis variabilis was investigated by using a minimal medium containing glucose as the carbon and energy source,
Purification and characterization of a nitrilase from Fusarium solani O1
Abstract An intracellular nitrilase was purified from a Fusarium solani O1 culture, in which the enzyme (up to 3000 U L −1 ) was induced by 2-cyanopyridine. SDS-PAGE revealed one major band
Quirks of Error Estimation in Cross-Linking/Mass Spectrometry
Cross-linking/mass spectrometry is an increasingly popular approach to obtain structural information on proteins and their complexes in solution. However, methods for error assessment are under
Galactosylation of antibiotics using the β-galactosidase from Aspergillus oryzae
Abstract The β-galactosidase from Aspergillus oryzae has been shown to catalyze the synthesis of β-galactosides of antibiotics such as chlorphenisin and chloramphenicol using β-lactose as the