• Publications
  • Influence
Enzymatic Synthesis of a Quorum-Sensing Autoinducer Through Use of Defined Substrates
Many bacteria, including several pathogens of plants and humans, use a pheromone called an autoinducer to regulate gene expression in a cell density-dependent manner. Agrobacterium autoinducer [AAI,Expand
  • 368
  • 23
Human Flap Endonuclease Structures, DNA Double-Base Flipping, and a Unified Understanding of the FEN1 Superfamily
Flap endonuclease (FEN1), essential for DNA replication and repair, removes RNA and DNA 5' flaps. FEN1 5' nuclease superfamily members acting in nucleotide excision repair (XPG), mismatch repairExpand
  • 200
  • 20
  • PDF
Mutations linked to dyskeratosis congenita cause changes in the structural equilibrium in telomerase RNA
Autosomal dominant dyskeratosis congenita (DKC), as well as aplastic anemia, has been linked to mutations in the RNA component of telomerase, the ribonucleoprotein responsible for telomereExpand
  • 157
  • 8
The 3′-Flap Pocket of Human Flap Endonuclease 1 Is Critical for Substrate Binding and Catalysis*
Flap endonuclease 1 (FEN1) proteins, which are present in all kingdoms of life, catalyze the sequence-independent hydrolysis of the bifurcated nucleic acid intermediates formed during DNA replicationExpand
  • 51
  • 8
Multiple but dissectible functions of FEN‐1 nucleases in nucleic acid processing, genome stability and diseases
Flap EndoNuclease‐1 (FEN‐1) is a multifunctional and structure‐specific nuclease involved in nucleic acid processing pathways. It plays a critical role in maintaining human genome stability throughExpand
  • 127
  • 7
Flap endonucleases pass 5′-flaps through a flexible arch using a disorder-thread-order mechanism to confer specificity for free 5′-ends
Flap endonucleases (FENs), essential for DNA replication and repair, recognize and remove RNA or DNA 5′-flaps. Related to FEN specificity for substrates with free 5′-ends, but controversial, is theExpand
  • 40
  • 5
The DNA–protein interaction modes of FEN-1 with gap substrates and their implication in preventing duplication mutations
Flap endonuclease-1 (FEN-1) is a structure-specific nuclease best known for its involvement in RNA primer removal and long-patch base excision repair. This enzyme is known to possess 5′-flap endo-Expand
  • 39
  • 4
  • PDF
The wonders of flap endonucleases: structure, function, mechanism and regulation.
Processing of Okazaki fragments to complete lagging strand DNA synthesis requires coordination among several proteins. RNA primers and DNA synthesised by DNA polymerase α are displaced by DNAExpand
  • 38
  • 4
  • PDF
Solution structure of the complex formed by the two N-terminal RNA-binding domains of nucleolin and a pre-rRNA target.
Nucleolin is a 70 kDa multidomain protein involved in several steps of eukaryotic ribosome biogenesis. In vitro selection in combination with mutagenesis and structural analysis identified bindingExpand
  • 54
  • 3
Unpairing and gating: sequence-independent substrate recognition by FEN superfamily nucleases.
Structure-specific 5'-nucleases form a superfamily of evolutionarily conserved phosphodiesterases that catalyse a precise incision of a diverse range of DNA and RNA substrates in aExpand
  • 51
  • 3