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Optogenetic dissection of mitotic spindle positioning in vivo
TLDR
The results define Gα∙GDP–GPR-1/2Pins/LGN as a regulatable membrane anchor, and LIN-5Mud/NuMA as a potent activator of dynein-dependent spindle-positioning forces. Expand
Two populations of cytoplasmic dynein contribute to spindle positioning in C. elegans embryos
TLDR
Fluorescently labeling endogenous proteins in Caenorhabditis elegans embryos show that dynein exists in two distinct cortical populations that directly depends on LIN-5, whereas the other is concentrated at MT plus ends and depends on end-binding proteins. Expand
Tumor suppressor APC is an attenuator of spindle-pulling forces during C. elegans asymmetric cell division
TLDR
The results document a physical basis for the attenuation of spindle-pulling force, which may be generally used in asymmetric cell division and, when disrupted, potentially contributes to division defects in cancer. Expand
Multisite Phosphorylation of NuMA-Related LIN-5 Controls Mitotic Spindle Positioning in C. elegans
TLDR
Evidence is provided that phosphorylation of multiple LIN-5 domains by different kinases contributes to a mechanism for spatiotemporal control of spindle positioning and chromosome segregation and a novel strategy to characterize early embryonic defects in lethal T168,T181 knockin substitution mutants is applied. Expand
Optogenetic dissection of mitotic spindle positioning in vivo
TLDR
This results define Gα∙GDP–GPR-1/2Pins/LGN as a regulatable membrane anchor, and LIN-5Mud/NuMA as a potent activator of dynein-dependent spindle positioning forces, and highlights the possibilities for optogenetic control of endogenous proteins within an animal system. Expand
The tumor suppressor APC is an attenuator of spindle-pulling forces during C. elegans asymmetric cell division
TLDR
This study is the first to document a mechanical role for the APC protein, and provides a physical basis for spindle-pulling force attenuation. Expand
Pulling apart mitotic spindle positioning in the C. elegans early embryo
Development and homeostasis of tissues and organisms depend on correct execution of cell division. To control the size, content, and position of daughter cells, the mother cell’s cleavage planeExpand
Abstract 126: HUB Organoids: bringing the 'patient in the lab' for preclinical and clinical development
TLDR
HUB Organoids could add a functional test for therapy response in a patient centered setting providing a unique tool for personalized medicine and identify novel targets, identify lead compounds and stratify potential responders based on their genetic or molecular profile. Expand
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