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The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins.
TLDR
These analyses confirm the taxonomic position of C. glutamicum as related to Mycobacteria and show a broad metabolic diversity as expected for a bacterium living in the soil.
Handbook of Corynebacterium glutamicum
Part I: History A Short History of the Birth of the Amino Acid Industry in Japan, S. Kinoshita Part II: Taxonomy Corynebacterium Taxonomy, W. Liebl Part III: Genome, Plasmids and Gene Expression The
Isoleucine synthesis in Corynebacterium glutamicum: molecular analysis of the ilvB-ilvN-ilvC operon
TLDR
The amounts of the ilvBNC and ilvNC transcripts increased in response to the addition of alpha-ketobutyrate to the growth medium, correlated to an increase in specific AHAS activity, whereas IR activity was not increased because of the relatively large amount of theIlvC transcript present under all conditions assayed.
Determination of the fluxes in the central metabolism of Corynebacterium glutamicum by nuclear magnetic resonance spectroscopy combined with metabolite balancing
TLDR
In vivo one component within these anaplerotic reactions is a back flux from the carbon 4 units of the tricarboxylic acid cycle to the carbon 3 units of glycolysis of 30.6% and the total net flux of the anaplerosis reactions was quantitated as 38.0%.
A high-throughput approach to identify genomic variants of bacterial metabolite producers at the single-cell level
TLDR
A novel method for visualizing intracellular metabolite concentrations within single cells of Escherichia coli and Corynebacterium glutamicum that expedites the screening process of producers and fills the gap between existing high-throughput methods for mutant generation and genome analysis.
A new type of transporter with a new type of cellular function: l‐lysine export from Corynebacterium glutamicum
TLDR
These results distinguish LysE as an exporter, which structurally represents a new type of translocator; demonstrates that exporters are also present for primary metabolites such as amino acids; and serves in one physiological function to link import with export activity.
Export of l-Isoleucine from Corynebacterium glutamicum: a Two-Gene-Encoded Member of a New Translocator Family
TLDR
Comparisons of the BrnF and BrnE phylogenetic trees show that gene duplication events in the early bacterial lineage gave rise to multiple paralogues that have been retained in alpha-proteobacteria but not in other prokaryotes analyzed.
Linking Central Metabolism with Increased Pathway Flux: l-Valine Accumulation by Corynebacterium glutamicum
ABSTRACT Mutants of Corynebacterium glutamicum were made and enzymatically characterized to clone ilvD and ilvE, which encode dihydroxy acid dehydratase and transaminase B, respectively. These genes
Characterization of a Corynebacterium glutamicum Lactate Utilization Operon Induced during Temperature-Triggered Glutamate Production
TLDR
Gene expression changes of glutamate-producing Corynebacterium glutamicum were identified in transcriptome comparisons by DNA microarray analysis, indicating that the function of the permease gene NCgl2816 is dispensable, while LldD is essential, for growth of C. glutamum on l-lactate.
Expression control and specificity of the basic amino acid exporter LysE of Corynebacterium glutamicum.
TLDR
The physiological role of the lysEG system is to prevent bacteriostasis due to elevated L-lysine or L-arginine concentrations that arise during growth in the presence of peptides or in mutants possessing a deregulated biosynthesis pathway.
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