Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88
- H. Pel, J. H. Winde, H. Stam
- BiologyNature Biotechnology
- 1 February 2007
The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for the production of enzymes and organic acids, particularly citric acid, and the sequenced genome revealed a large number of major facilitator superfamily transporters and fungal zinc binuclear cluster transcription factors.
Properties and applications of starch-converting enzymes of the α-amylase family
- M. Maarel, B. A. Veen, J. Uitdehaag, H. Leemhuis, L. Dijkhuizen
- 28 March 2002
A novel class of secreted hydrophobic proteins is involved in aerial hyphae formation in Streptomyces coelicolor by forming amyloid-like fibrils.
- D. Claessen, R. Rink, H. Wosten
- Biology, EngineeringGenes & Development
- 15 July 2003
It is concluded that the amyloid-like fibrils of ChpD-H lower the water surface tension to allow aerial growth and cover aerial structures, rendering them hydrophobic.
A gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into Mycobacterium tuberculosis survival in macrophages
- R. van der Geize, Katherine C. Yam, L. Eltis
- BiologyProceedings of the National Academy of Sciences
- 6 February 2007
The results suggest that cholesterol metabolism is central to M. tuberculosis's unusual ability to survive in macrophages and provide insights into potential targets for novel therapeutics.
Structure-Function Relationships of Glucansucrase and Fructansucrase Enzymes from Lactic Acid Bacteria
- S. V. van Hijum, S. Kralj, L. Ozimek, L. Dijkhuizen, Ineke H van Geel-Schutten
- BiologyMicrobiology and Molecular Biology Reviews
- 1 March 2006
Emphasis now is on identification of residues and regions important for GS enzyme activity and product specificity (synthesis of α-glucans differing in glycosidic linkage type, degree and type of branching, glucan molecular mass, and solubility).
Glucansucrases: three-dimensional structures, reactions, mechanism, α-glucan analysis and their implications in biotechnology and food applications.
- H. Leemhuis, T. Pijning, L. Dijkhuizen
- BiologyJournal of Biotechnology
- 20 January 2013
The Actinobacterial mce4 Locus Encodes a Steroid Transporter*
- W. Mohn, R. van der Geize, L. Eltis
- BiologyJournal of Biological Chemistry
- 19 December 2008
There is direct evidence that the Mce4 system of Rhodococcus jostii RHA1 is a steroid uptake system, and Bioinformatic analysis revealed that all mce4 loci in sequenced genomes are linked to steroid metabolism genes, predicting that all Mce 4 systems are steroid transporters.
Methanol metabolism in thermotolerant methylotrophic Bacillus strains involving a novel catabolic NAD-dependent methanol dehydrogenase as a key enzyme
- N. Arfman, E. Watling, L. Dijkhuizen
- BiologyArchives of Microbiology
Failure of whole cells to oxidize formate, and the absence of formaldehyde-and formate dehydrogenases indicated the operation of a non-linear oxidation sequence for formaldehyde via HPS, which suggested that the synthesis of these enzymes is not under coordinate control.
Nucleotide sequence and X-ray structure of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 in a maltose-dependent crystal form.
- C. L. Lawson, R. V. van Montfort, B. Dijkstra
- Biology, ChemistryJournal of Molecular Biology
- 18 February 1994
The cyclodextrin glycosyltransferase (CGTase, EC 126.96.36.199) gene from Bacillus circulans strain 251 was cloned and sequenced. It was found to code for a mature protein of 686 amino acid residues,…
Glucan synthesis in the genus Lactobacillus: isolation and characterization of glucansucrase genes, enzymes and glucan products from six different strains.
- S. Kralj, G. H. van Geel-Schutten, M. Dondorff, S. Kirsanovs, M. van der Maarel, L. Dijkhuizen
- Biology, MedicineMicrobiology
- 1 November 2004
For the first time, isolation and characterization of dextransucrase and mutansucrase genes and enzymes from various Lactobacillus species and the characterization of the glucan products synthesized are reported, which mainly have alpha-(1-->6)- and alpha-glucosidic linkages.