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Extremely Radiation-Resistant Mutants of a Halophilic Archaeon with Increased Single-Stranded DNA-Binding Protein (RPA) Gene Expression
Abstract DeVeaux, L. C., Müller, J. A., Smith, J. R., Petrisko, J., Wells, D. P. and DasSarma, S. Extremely Radiation-Resistant Mutants of a Halophilic Archaeon with Increased Single-StrandedExpand
Region-specific activators of meiotic recombination in Schizosaccharomyces pombe.
Schizosaccharomyces pombe rec mutants were previously isolated on the basis of their deficiency in meiotic recombination at the ade6 locus. We surveyed their meiotic recombination deficiencies at andExpand
Cloning, sequencing, and characterization of Escherichia coli thioesterase II.
The gene (tesB) encoding Escherichia coli thioesterase II, a low-abundance enzyme of unknown physiological function which can hydrolyze a broad range of acyl-CoA thioesters, has been localized byExpand
Biosynthetic radiolabeling of bacterial lipopolysaccharide to high specific activity.
We describe a method for producing radiolabeled lipopolysaccharide (LPS) by incorporating [3H]acetate into an aceEF, gltA strain of Escherichia coli K12. The LPS has substantially greater specificExpand
Metabolic engineering as a tool for enhanced lactic acid production.
Metabolic engineering is a powerful biotechnological tool that finds, among others, increased use in constructing microbial strains for higher lactic acid productivity, lower costs and reducedExpand
The Essential Role of the Deinococcus radiodurans ssb Gene in Cell Survival and Radiation Tolerance
Recent evidence has implicated single-stranded DNA-binding protein (SSB) expression level as an important factor in microbial radiation resistance. The genome of the extremely radiation resistantExpand
Accelerator-based radiation sources for next-generation radiobiological research
Abstract The Idaho Accelerator Center (IAC) of Idaho State University has developed a unique radiation research facility to answer next-generation radiobiological questions. The IAC has 10 operatingExpand
Concurrent metabolism of pentose and hexose sugars by the polyextremophile Alicyclobacillus acidocaldarius
Alicyclobacillus acidocaldarius is a thermoacidophilic bacterium capable of growth on sugars from plant biomass. Carbon catabolite repression (CCR) allows bacteria to focus cellular resources on aExpand
Genomic replacement in Escherichia coli K-12 using covalently closed circular plasmid DNA.
A number of gene replacements at different loci were constructed using covalently closed circular (ccc) plasmid DNA in the recB21 recC22 sbcB15 sbcC201 mutant of Escherichia coli (JC7623). SelectedExpand
Meiotic chromosome dynamics dependent upon the rec8(+), rec10(+) and rec11(+) genes of the fission yeast Schizosaccharomyces pombe.
During meiosis homologous chromosomes replicate once, pair, experience recombination, and undergo two rounds of segregation to produce haploid meiotic products. The rec8(+), rec10(+), and rec11(+)Expand