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Ribosomal Protein L23 Activates p53 by Inhibiting MDM2 Function in Response to Ribosomal Perturbation but Not to Translation Inhibition
Ectopic expression of L23 reduced MDM2-mediated p53 ubiquitination and also induced p53 activity and G1 arrest in p 53-proficient U2OS cells but not in p53-deficient Saos-2 cells, revealing that L23 is another regulator of the p53 -MDM2 feedback regulation. Expand
Aldosterone mediates activation of the thiazide-sensitive Na-Cl cotransporter through an SGK1 and WNK4 signaling pathway.
Measurement of sodium uptake in a Xenopus oocyte expression system found that serum and glucocorticoid-induced kinase 1 (SGK1), an aldosterone-responsive gene expressed in the DN, attenuated the inhibitory effect of WNK4 on NCC activity. Expand
The R109H Variant of Fascin-2, a Developmentally Regulated Actin Crosslinker in Hair-Cell Stereocilia, Underlies Early-Onset Hearing Loss of DBA/2J Mice
The quantitative trait locus ahl8 is a key contributor to the early-onset, age-related hearing loss of DBA/2J mice. A nonsynonymous nucleotide substitution in the mouse fascin-2 gene (Fscn2) isExpand
Identification of protein modifications using MS/MS de novo sequencing and the OpenSea alignment algorithm.
It is determined that a mass-based alignment algorithm (OpenSea) for de novo sequencing results could identify post-translationally modified peptides in a high-throughput environment using automated data processing algorithms such as OpenSea. Expand
Lens proteomics: the accumulation of crystallin modifications in the mouse lens with age.
2-DE proteome maps of mouse lens proteins created in this study will be useful for comparison with maps of lens proteins of mice with cataracts so that cataract-specific modifications may be identified. Expand
Age-related changes in human lens crystallins identified by two-dimensional electrophoresis and mass spectrometry.
The results provided the most detailed map to date of the identities of protein species on two-dimensional electrophoresis gels of adult human lenses, indicating that a major fraction of the water-soluble protein of the adult human lens is composed of truncated beta B1 and beta A3/A1 crystallins, and that nearly all human crystallins are susceptible to deamidation. Expand
Age-related changes in human crystallins determined from comparative analysis of post-translational modifications in young and aged lens: does deamidation contribute to crystallin insolubility?
This work employed recently developed blind modification search techniques to generate the most comprehensive map of post-translational modifications in human lens constructed to date and found that the extent of deamidation was significantly increased in the water-insoluble fractions of the aged lenses. Expand
Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits.
It is demonstrated that mammalian OST can be isolated from solubilized, actively engaged ribosomes as multiple distinct protein complexes that range in size from approximately 500 to 700 kDa, and a remarkable heterogeneity in OST composition that may reflect a means for controlling nascent chain glycosylation. Expand
γN‐crystallin and the evolution of the βγ‐crystallin superfamily in vertebrates
Although well conserved throughout vertebrate evolution, γN in primates has apparently undergone major changes and possible loss of functional expression, and thus appear to be the ‘missing link’ between the β and γ crystallin lineages. Expand
High-throughput identification of proteins and unanticipated sequence modifications using a mass-based alignment algorithm for MS/MS de novo sequencing results.
A novel, mass-based approach to sequence alignment, implemented as a program called OpenSea, which can identify more peptides and proteins than commonly used database-searching programs while accurately locating sequence variation sites and unanticipated posttranslational modifications in a high-throughput environment. Expand