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Factors affecting lipid accumulation by Nannochloropsis oculata in a two-stage cultivation process
A two-stage cultivation strategy to enhance lipid production of the microalga Nannochloropsis oculata with respect to different levels of inoculum concentration, salinity of culture broth, and intensity of irradiance is proposed. Expand
High yield bio-butanol production by solvent-producing bacterial microflora.
The medium composition for bio-butanol production was optimized using the Box-Behnken design and response surface methodology (RSM) and the effects of glucose, FeSO(4) · 7H(2)O and yeast extract concentrations on the butanol production by the mixture culture were investigated on batch mode. Expand
Hyaluronic acid production by recombinant Lactococcus lactis
HA was made possible to be produced by a generally recognized as safe Lactococcus lactis by coexpressing HA synthase and uridine diphosphate–glucose dehydrogenase (UDP-GlcDH) of Streptococcus equi subsp. Expand
Enhanced Hyaluronic Acid Production in Bacillussubtilis by Coexpressing Bacterial Hemoglobin
Although HA production could be achieved by expressing hasA alone, coexpressing hasB or tauD with hasA could enhance HA production at least 2‐fold, and to replenish the energy consumed for HA biosynthesis, Vitreoscilla hemoglobin was coexpressed with the HA‐expressing genes. Expand
Enhancement of Cellulose Pellicle Production by Constitutively Expressing Vitreoscilla Hemoglobin in Acetobacter xylinum
Vitreoscilla hemoglobin (VHb) gene driven by the constitutive bla promoter was expressed in the cellulose‐producing Acetobacter xylinum. The expressed VHb was biochemically active and could enhanceExpand
Accumulation of lipid production in Chlorella minutissima by triacylglycerol biosynthesis-related genes cloned from Saccharomyces cerevisiae and Yarrowia lipolytica
This approach can be used with the biodiesel production platform of C. minutissima UTEX 2219 for high lipid production that will, in turn, enhance productivity, and indeed it was found this approach indeed increased the storage lipid content of Biodiesel up to 2-fold over that of wild type. Expand
Expression of Bacterial Hemoglobin in the Yeast, Pichia pastoris, with a Low O2-Induced Promoter
The application of O2-regulated promoter in P. pastoris revealed that induction of high-level expression of heterologous protein is feasible without addition of supplementary compounds. Expand
Novel Codon-optimization Genes Encoded in Chlorella for Triacylglycerol Accumulation☆
This work provides the optimization of TAG production in Chlorella sp. Expand
Biosilicification of dual‐fusion enzyme immobilized on magnetic nanoparticle
This process provides the advantages that recombinant fusion enzyme can be directly recovered from crude extract, silica encapsulation protects the enzyme from leakage and denaturation, and the enzyme activity can be easily retrieved by applying a magnetic field. Expand
Expressing Vitreoscilla hemoglobin in statically cultured Acetobacter xylinum with reduced O(2) tension maximizes bacterial cellulose pellicle production.
Vitreoscilla hemoglobin (VHb) was constitutively expressed in Acetobacter xylinum to enhance bacterial cellulose (BC) production. A pronounced enhancement of BC production in static culture wasExpand