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DNA primase. Processivity and the primase to polymerase alpha activity switch.
Calf thymus DNA primase was examined to determine the kinetic parameters that define its unusual processivity. At 37 degrees C, the major products were 8-9 and 2-3 nucleotides long. The 2-mer was the… Expand
Multiple roles of divalent cation in the terminal deoxynucleotidyltransferase reaction.
Terminal deoxynucleotidyltransferase activity is absolutely dependent on the presence of a divalent cation in the reaction mixture. This requirement can be satisfied by either Mg2+, Co2+, or Mn2+.… Expand
Development of terminal deoxynucleotidyl transferase activity in embryonic calf thymus gland.
- L. Chang
- Biology, Medicine
- Biochemical and biophysical research…
- 2 July 1971
Summary Analysis of extracts of calf embryo tissues for deoxynucleotide polymerizing enzymes showed that terminal deoxynucleotidyl transferase is present only in thymus and the levels of activity… Expand
Low molecular weight deoxyribonucleic acid polymerase from rabbit bone marrow.
Molecular biology of terminal transferase.
Terminal transferase is an unusual deoxynucleotide polymerizing enzyme found only in prelymphocytes. The protein was purified to homogeneity from calf thymus glands in 1971 as a 32 kDa protein with a… Expand
Induction of DNA polymerase in mouse L cells.
Abstract Two molecular species of DNA polymerase are found in mouse L cells. This study is concerned with the variation of these two species of enzyme with the rate of cell growth and DNA synthesis.… Expand
Low molecular weight deoxyribonucleic acid polymerase in mammalian cells.
Abstract Extracts of mammalian cells contain a 3.3 S deoxynucleotidyltransferase activity in addition to the 6 to 8 S DNA polymerase. The 3.3 S enzyme extracted from rabbit bone marrow uses template… Expand
Antigenic Relationships in Mammalian DNA Polymerase
Rabbit antibody was prepared against a high-molecular-weight DNA polymerase purified from the soluble fraction of calf thymus gland. This antibody does not inhibit terminal deoxynucleotidyl… Expand
Isolation of the gene encoding yeast DNA polymerase I
A yeast genomic DNA expression library in lambda gt11 antibody prepared against yeast DNA polymerase I were used to isolate the gene encoding DNA polymerase I. The identity of the DNA polymerase I… Expand
Expression of human terminal deoxynucleotidyl transferase in Escherichia coli.
- R. Peterson, L. Cheung, R. Mattaliano, S. White, L. Chang, F. J. Bollum
- Biology, Medicine
- The Journal of biological chemistry
- 5 September 1985
A cloned DNA fragment related to pT17 containing a partial cDNA sequence of human terminal deoxynucleotidyl transferase was used as a probe to screen for the full length cDNA sequence of the enzyme… Expand