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Molecular and Morphological Characterization of an Unusual Meloidogyne arenaria Population from Traveler's Tree, Ravenala madagascariensis.
No morphological or molecular features clearly distinguished this population as a new species, and, when considered as a whole, the evidence points to its identification as M. arenaria.
Amplification of Hsp90 homologs from plant-parasitic nematodes using degenerate primers and ramped annealing PCR.
RAN-PCR, which the annealing temperature is gradually reduced within each PCR cycle, led to the successful amplification of heat shock protein genes from nematode genomic DNA using degenerate primers and may be generally applicable for degenerate PCR on complex, uncharacterized templates.
Improved 18S small subunit rDNA primers for problematic nematode amplification
New primers were developed based on a very large 276 taxon alignment of 124 agriculturally important nematodes species, and tested on problematic nematode taxa such as Aphelenchoides, Bursaphelenchus, Ditylenchus, and Panagrolaimus for successful generation of sequences useful in future investigations of Nematode systematics.
Intercellular signaling during Caenorhabditis elegans vulval induction.
A trichodorus (triplonchida: trichodoridae) nematode from thrips (thysanoptera: panchaetothripinae).
A thrips insect Caliothrips sp. (Thysanoptera: Panchaetothripinae) from persimmon fruit (Ebenaceae: Diospyros sp.) from an unknown origin, possibly Asia, was intercepted in a passenger bag in…
PCR amplification of a long rDNA segment with one primer pair in agriculturally important nematodes
A strategy for generating all these molecular sequences at once through PCR amplification of a large ribosomal 3.3 to 4.2 kb DNA target was developed and the value of this strategy was demonstrated, the long rDNA segment was amplified and directly sequenced in 17 agriculturally important nematodes from the Tylenchida, Aphelenchida and Dorylaimida.