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Resonance energy transfer: methods and applications.
Resonance energy transfer is widely used in studies of biomolecular structure and dynamics. It provides information about distances on the order of 10 to 100 A and is thus suitable for investigating… Expand
Time-resolved fluorescence of proteins.
The Global Analysis of Fluorescence Intensity and Anisotropy Decay Data: Second-Generation Theory and Programs
Data registration and selective single-molecule analysis using multi-parameter fluorescence detection.
- C. Eggeling, S. Berger, +4 authors C. Seidel
- Chemistry, Medicine
- Journal of biotechnology
- 13 April 2001
A general strategy to identify and quantify sample molecules in dilute solution employing a new spectroscopic method for data registration and specific burst analysis denoted as multi-parameter… Expand
Fluorescence probes for structure.
Simultaneous analysis of multiple fluorescence decay curves: A global approach
Abstract A procedure is described for simultaneous analysis of multiple fluorescence decay curves. The advantage of this approach is that it exploits relationships between individual decays. Analysis… Expand
Profluorescent protease substrates: intramolecular dimers described by the exciton model.
- B. Packard, D. Toptygin, A. Komoriya, L. Brand
- Chemistry, Medicine
- Proceedings of the National Academy of Sciences…
- 15 October 1996
Xanthene dyes are known to form dimers with spectral characteristics that have been interpreted in terms of exciton theory. A unique aspect of H-type dimers is the fluorescence quenching that… Expand
The effects of conformational heterogeneity on the binding of the Notch intracellular domain to effector proteins: a case of biologically tuned disorder.
- A. Bertagna, D. Toptygin, L. Brand, D. Barrick
- Biology, Medicine
- Biochemical Society transactions
- 1 April 2008
Cell-fate decisions in metazoans are frequently guided by the Notch signalling pathway. Notch signalling is orchestrated by a type-1 transmembrane protein, which, upon interacting with extracellular… Expand
Analysis of fluorescence decay curves by means of the Laplace transformation.
A computational procedure is described for the analysis of fluorescence decay data convolved with a lamp flash of finite width. The computer program calculates the ratio of the Laplace transforms of… Expand
Fluorescence decay studies of reduced nicotinamide adenine dinucleotide in solution and bound to liver alcohol dehydrogenase.
The monophoton counting technique was used to obtain the fluorescence decay kinetics of NADH (dihydronicotinamide adenine dinucleotide) bound to LADH (HORSE LIVER ALCOHOL DEHYDROGENAS). It was found… Expand