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Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
There continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes, so it is important to update guidelines for monitoring autophagic activity in different organisms. Expand
KIT mutation in mast cells and other bone marrow hematopoietic cell lineages in systemic mast cell disorders: a prospective study of the Spanish Network on Mastocytosis (REMA) in a series of 113
The results would support the notion that KIT mutation is a hallmark of adult SM where it targets a pluripotent hematopoietic stem cell, and may contribute to explaining previously observed discrepancies in the literature. Expand
First toxicity report of tetrodotoxin and 5,6,11-trideoxyTTX in the trumpet shell Charonia lampas lampas in Europe.
This paper shows for first time the presence and toxicity of TTX and 5,6,11-trideoxyTTX in a trumpet shell collected in the European coasts and the results obtained by mouse bioassay showed that the analogue is much less toxic than TTX. Expand
First toxin profile of ciguateric fish in Madeira Arquipelago (Europe).
Analysis of fish samples from Madeira Arquipelago confirmed the presence of ciguatera fish poisoning toxins known as ciguatoxins (CTXs) for the first time in fish from this genus. Expand
Liquid chromatography–mass spectrometry method to detect Tetrodotoxin and Its analogues in the puffer fish Lagocephalus sceleratus (Gmelin, 1789) from European waters
Abstract The presence of tetrodotoxin (TTX) and its analogues, 4-epiTTX, 4,9-anhydroTTX, 5-deoxyTTX, 11-deoxyTTX, 5,6,11-trideoxyTTX, 11-norTTX-6(S)-ol, and 11-norTTX-6(R)-ol was investigated for theExpand
Comparative study of the stability of saxitoxin and neosaxitoxin in acidic solutions and lyophilized samples.
This paper analysed the stability of saxitoxin (STX) and neosaxitoxin in acidic solution and lyophilized samples to demonstrate that STX is very stable in solution samples and could be adopted as a reference standard. Expand
Risks for public health related to the presence of tetrodotoxin (TTX) and TTX analogues in marine bivalves and gastropods
Liquid chromatography with tandem mass spectroscopy methods are the most suitable for identification and quantification of TTX and its analogues, with LOQs between 1 and 25 μg/kg, and Estimated relative potencies for analogues are lower than that ofTTX but are associated with a high degree of uncertainty. Expand
In vitro and in vivo evaluation of paralytic shellfish poisoning toxin potency and the influence of the pH of extraction.
An in vitro quantification method is developed using cultured neurons and the first data evaluating the potencies of PSP toxins after extraction in acidic pHs are provided, indicating that the toxicity of the sample increases in acidic conditions. Expand
Marine toxins and the cytoskeleton: okadaic acid and dinophysistoxins
OA and dinophysistoxin‐1 (35S‐methylokadaic acid) cause rapid changes in the structural organization of intermediate filaments, followed by a loss of microtubules, solubilization of intermediate filament proteins, and disruption of desmosomes. Expand
Cytoskeletal disruption is the key factor that triggers apoptosis in okadaic acid-treated neuroblastoma cells
Results demonstrate that collapse of mitochondrial membrane potential is accessory in the execution of apoptosis, which is directly dependent on cytoskeletal changes. Expand