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MIA40 is an oxidoreductase that catalyzes oxidative protein folding in mitochondria
TLDR
MIA40 represents a thioredoxin-unrelated, minimal oxidoreductase, with a facile CPC redox active site that ensures its catalytic function in oxidative folding in mitochondria.
Structure of human Wilson protein domains 5 and 6 and their interplay with domain 4 and the copper chaperone HAH1 in copper uptake.
TLDR
Human Wilson protein is a copper-transporting ATPase located in the secretory pathway possessing six N-terminal metal-binding domains, which are suggested to be two acceptors of Cu(I) from HAH1, which then somehow route copper to WLN5-6, before the ATP-driven transport of copper across the vesicular membrane.
Counting the zinc-proteins encoded in the human genome.
TLDR
The present bioinformatic research proposes a strategy to answer the question of how many and which proteins encoded in the human genome may require zinc for their physiological function by a combination of approaches, which include searching in the proteome for the zinc-binding patterns that are obtained from all available X-ray data.
Opposing cardioprotective actions and parallel hypertrophic effects of δPKC and ɛPKC
TLDR
It is demonstrated that two related PKC isozymes have both parallel and opposing effects in the heart, indicating the danger in the use of therapeutics with nonselective isozyme inhibitors and activators.
Mitochondrial copper(I) transfer from Cox17 to Sco1 is coupled to electron transfer
TLDR
It is shown here that Cu(I)HCox172S-S, i.e., the copper-loaded form of the protein, can transfer simultaneously copper( I) and two electrons to the human cochaperone Sco1 (HSco1) in the oxidized state, with its metal-binding cysteines forming a disulfide bond.
Affinity gradients drive copper to cellular destinations
TLDR
This study provides the thermodynamic basis for the kinetic processes that lead to the distribution of cellular copper, and complements the finding that fast copper-transfer pathways require metal-mediated protein– protein interactions and therefore protein–protein specific recognition.
Opposing cardioprotective actions and parallel hypertrophic effects of delta PKC and epsilon PKC.
TLDR
It is demonstrated that two related PKC isozymes have both parallel and opposing effects in the heart, indicating the danger in the use of therapeutics with nonselective isozyme inhibitors and activators.
Faster superoxide dismutase mutants designed by enhancing electrostatic guidance
TLDR
It is shown that site-specific mutants that increase local positive charge while maintaining this orienting network (Glu→Gin) have faster reaction rates and increased ionic-strength dependence, matching brownian dynamics simulations incorporating electrostatic terms.
A Structural-Dynamical Characterization of Human Cox17*
TLDR
Redox properties of the disulfides of human Cox17, here investigated, strongly support the current hypothesis that the unstructured fully reduced Cox17 protein is present in the cytoplasm and enters the intermembrane space (IMS) where is then oxidized by Mia40 to Cox172S-S, thus becoming partially structured and trapped into the IMS.
Metallochaperones and metal-transporting ATPases: a comparative analysis of sequences and structures.
TLDR
It is found that metallochaperones and their physiological partner ATPases from several phylogenetic kingdoms recognize one another, via an interplay of electrostatics, hydrogen bonding, and hydrophobic interactions, in a manner that precisely orients the metal-binding side chains for rapid metal transfer between otherwise tight binding sites.
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