Attachment to and biofilm formation on abiotic surfaces by Acinetobacter baumannii: involvement of a novel chaperone-usher pili assembly system.
- A. Tomaras, C. W. Dorsey, R. Edelmann, L. Actis
- BiologyMicrobiology
- 1 December 2003
Results demonstrate that the expression of a chaperone-usher secretion system, some of whose components appear to be acquired from unrelated sources, is required for pili formation and the concomitant attachment to plastic surfaces and the ensuing formation of biofilms by A. baumannii cells.
The Acinetobacter baumannii 19606 OmpA Protein Plays a Role in Biofilm Formation on Abiotic Surfaces and in the Interaction of This Pathogen with Eukaryotic Cells
- J. Gaddy, A. Tomaras, L. Actis
- BiologyInfection and Immunity
- 26 May 2009
It is shown that although outer membrane protein A (OmpA) of A. baumannii 19606 plays a partial role in the development of robust biofilms on plastic, it is essential for bacterial attachment to Candida albicans filaments and A549 human alveolar epithelial cells.
Acinetobacter baumannii: human infections, factors contributing to pathogenesis and animal models.
- M. McConnell, L. Actis, J. Pachón
- BiologyFEMS Microbiology Reviews
- 1 March 2013
This review summarizes the characteristics of A. baumannii that contribute to its pathogenesis, with a focus on motility, adherence, biofilm formation, and iron acquisition.
Characterization of a two-component regulatory system from Acinetobacter baumannii that controls biofilm formation and cellular morphology.
- A. Tomaras, M. J. Flagler, C. W. Dorsey, J. Gaddy, L. Actis
- BiologyMicrobiology
- 1 November 2008
Results indicate that BfmR is part of a two-component regulatory system that plays an important role in the morphology of A. baumannii 19606 cells and their ability to form biofilms on abiotic surfaces.
The Opportunistic Human Pathogen Acinetobacter baumannii Senses and Responds to Light
It is shown that the opportunistic pathogen Acinetobacter baumannii senses and responds to blue light, and the A1S_2225 gene, which codes for an 18.6-kDa protein that contains an N-terminal blue-light-sensing-using flavin (BLUF) domain and lacks a detectable output domain(s), was named blue-lightsensing A (blsA).
Regulation of Acinetobacter baumannii biofilm formation.
Interestingly, the BfmRS system also controls cell morphology under particular culture conditions, and this transcriptional regulatory system activates the expression of the usher-chaperone assembly system responsible for the production of pili, needed for cell attachment and biofilm formation on polystyrene surfaces.
AB5075, a Highly Virulent Isolate of Acinetobacter baumannii, as a Model Strain for the Evaluation of Pathogenesis and Antimicrobial Treatments
- Anna C. Jacobs, M. Thompson, D. Zurawski
- Biology, MedicinemBio
- 27 May 2014
It is proposed that AB5075 can serve as a model strain for A. baumannii pathogenesis due to its relatively recent isolation, multidrug resistance, reproducible virulence in animal models, and genetic tractability.
Acinetobacter baumannii Strain M2 Produces Type IV Pili Which Play a Role in Natural Transformation and Twitching Motility but Not Surface-Associated Motility
- Christian M. Harding, Erin N. Tracy, Michael D. Carruthers, P. Rather, L. Actis, R. Munson
- BiologymBio
- 6 August 2013
It is demonstrated that A. baumannii strain M2 is naturally transformable and capable of twitching motility, two classical TFP-associated phenotypes, and that surface-associated motility was not dependent on the products of the pilA, pilD, and pilT genes and, by correlation, TFP.
Biofilm formation in Acinetobacter baumannii: associated features and clinical implications.
- J. RodrÃguez-Baño, S. MartÃ, J. Vila
- Biology, MedicineClinical Microbiology and Infection
- 1 March 2008
Multivariate analysis revealed that treatment in an intensive care unit, ciprofloxacin resistance and isolation from a respiratory sample were associated with non-biofilm-forming isolates, while previous aminoglycoside use was associated with biofilm-formed isolates.
Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
- W. Pope, Charles A. Bowman, Po-Cheng Tang
- BiologyeLife
- 28 April 2015
To understand the population structure of phages infecting a single host strain, 627 phages of Mycobacterium smegmatis were isolated, sequenced, and compared to reveal a continuum of genetic diversity, albeit with uneven representation of different phages.
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